> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/advanced/advanced-sample-preparation.md).

# Advanced sample preparation

- [Prepare your sample](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/prepare-your-sample.md)
- [Break up clumps and chains](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/break-up-clumps-and-chains.md)
- [Disaggregate mechanically](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/break-up-clumps-and-chains/disaggregate-mechanically.md)
- [Disaggregate with surfactant](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/break-up-clumps-and-chains/disaggregate-with-surfactant.md)
- [Avoid cell lysis](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/break-up-clumps-and-chains/avoid-cell-lysis.md): Sample over time to investigate effectiveness and absence of cell lysis
- [Remove large particles](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/remove-large-particles.md)
- [Hit the right concentration](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration.md)
- [Dilute 1:10](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-10.md)
- [Dilute 1:100](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-100.md)
- [Dilute 1:1 000](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-1-000.md)
- [Dilute 1:10 000](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-10-000.md)
- [Dilute 1:100 000](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-100-000.md)
- [Concentrate your sample](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/concentrate-your-sample.md): It is generally not recommended to use BactoBox® for measuring samples with very low bacterial concentrations. This page is a collection of tips and tricks for how to do so.
- [Filter-centrifuge your sample](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/concentrate-your-sample/filter-centrifuge-your-sample.md)
- [Filter-centrifuge your sample with Vivaspin® 20](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/concentrate-your-sample/filter-centrifuge-your-sample/filter-centrifuge-your-sample-with-vivaspin-r-20.md)
- [Centrifuge your sample](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/concentrate-your-sample/centrifuge-your-sample.md)
- [Hit the right conductivity](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-conductivity.md)
- [Make diluent](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-conductivity/make-diluent.md): The Do-It-Yourself (DIY) guide to make diluent
- [Measure conductivity with BactoBox®](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-conductivity/measure-conductivity-with-bactobox-r.md)
- [Focus on target objects](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/focus-on-target-objects.md)
- [Measure background particulates](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/focus-on-target-objects/measure-particulates-in-growth-medium.md)
- [Is background substantial?](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/focus-on-target-objects/is-background-substantial.md)
- [Mitigate high background](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/focus-on-target-objects/mitigate-high-background.md)
- [Summary](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/focus-on-target-objects/summary.md)
- [Make sample vial](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/make-sample-vial.md)


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://help.sbtinstruments.com/advanced/advanced-sample-preparation.md?ask=<question>&goal=<endgoal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.