> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-10.md).

# Dilute 1:10

Get a [dilution vial](/item-register/individual-items/dilution-vial.md). Discard 1 mL of diluent so that the dilution vial is filled to the 9 mL mark.

Add 1 mL of your sample to the dilution vial (which brings it back up to the 10 mL mark). This results in a 1:10 dilution of your sample. Once mixed together, we call it your *sample vial* from now on.

![Procedure to make 1:10 dilutions](https://2483617760-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FCaBGkox4Fd7NCFFCF47X%2Fuploads%2Fgit-blob-a05724ba262ccf26b1c48bf6ad1361faf9e3fd47%2Fdilution--1-10-then-measure-with-bactobox.png?alt=media)

{% hint style="success" %}

## Vortex each dilution step thoroughly!

Always vortex thoroughly for at least **10 seconds**. Ensure that a clear cyclone/vortex appears.

You must perform proper vortexing to ensure that the transferred bacteria are well-mixed in the prepared dilution. Without proper vortexing, you may get underestimation of concentration and variation between replicates.
{% endhint %}

{% hint style="warning" %}

## Your sample vial is for *single use* only

Once you run the liquid through BactoBox®, the properties change *significantly*.

**Always discard your sample vial after use!**
{% endhint %}

## Is the final dilution factor in your sample vial 1:10?

You might run into conductivity-related errors. Avoid these with a [custom diluent](/advanced/advanced-sample-preparation/hit-the-right-conductivity.md#i-dilute-1-10-or-less).


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