> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-10-000.md).
# Dilute 1:10 000
Get two dilution vials. [Dilute 1:100](/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-100.md) into your first [Dilution vial](/item-register/individual-items/dilution-vial.md), and then [Dilute 1:100](/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-100.md) into your second dilution vial.
{% hint style="success" %}
## Vortex each dilution step thoroughly!
Always vortex thoroughly for at least **10 seconds**. Ensure that a clear cyclone/vortex appears.
You must perform proper vortexing to ensure that the transferred bacteria are well-mixed in the prepared dilution. Without proper vortexing, you may get underestimation of concentration and variation between replicates.
{% endhint %}
{% hint style="info" %}
**Preparing a dilution series with 1:100, 1:1,000 and 1:10,000 dilutions**
The most convenient way of preparing three samples diluted by 1:100, 1:1,000 and 1:10,000 is listed below.
Sample #1 - target dilution 1:100 [Dilute 1:100](/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-100.md) from primary sample.
Sample #2 - target dilution 1:1,000: [Dilute 1:10](/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-10.md) from sample #1. Also, see [Dilute 1:1 000](/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-1-000.md)
Sample #3 - target dilution 1:10,000: [Dilute 1:100](/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-100.md) from sample #1. Also, see [Dilute 1:10 000](/advanced/advanced-sample-preparation/hit-the-right-concentration/dilute-1-10-000.md)
{% endhint %}
---
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