Custom gating
We support custom gating settings through a custom solution.
Advanced feature 🧩
This is an advanced feature. We only support advanced features through a custom solution. Contact SBT for details.
Strain-specific gating
Legacy strain-specific gating
In the past, we offered custom gating settings to for specific bacteria species. While this worked in some use cases, the approach is too brittle for general use. There are simply too many combinations of bacterial species, sample preparation, media types, culture conditions, etc.
Use BacTotal
Use BacTotal if in doubt
BacTotal fits most use cases. It is also the default gating for BactoBox®.
The other gating settings are for legacy purposes.
BactoBox® (using BacTotal) counts every whole cell in a sample
Examples:
A whole cell that has stopped dividing counts towards cells/mL
A whole cell that no longer forms a colony counts towards cells/mL
A whole cell that takes up propidium iodide counts towards cells/mL
The one thing that removes a cell from the cells/mL count is full lysis. Because a cell that has come apart is no longer there.
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