When you relocate your BactoBox® setup.
Example: You install your BactoBox® for the very first time.
Example: You ship your BactoBox® to another physical location.
Example: You get your BactoBox® back from service/repair at SBT.
Difference between QC test and qualification
When you qualify your BactoBox® setup, always use an SBT dilution vial (bundled as DV01).
For the regular (weekly) QC test, use the dilution vial that you intend to use going forward for your own measurements. This depends on your use case and may be either of:
When you change your BactoBox® setup.
Example: You replace the flow cell.
Example: You replace the external filter.
Example: You replace the tubing kit.
When you switch from the SBT dilution vial to the DIY dilution vial.
After 30+ minutes of inactivity.
The BactoBox® software automatically prompts you to do this.
Why clean?
Clean serves two purposes: Avoid growth inside your BactoBox® and avoid carry-over effects between measurements.
Clean your BactoBox® to ensure that you only measure on your sample itself—not any residual particles or growth from previous measurements. E.g., to avoid carry-over, biofilm, and the like.
Your BactoBox® has microfluidic flow paths that the liquid, bacteria, and particles pass through. It's essential to keep these paths clear of residue, microorganisms, biofilm, and other contaminants. In general, to avoid conditions that promote microbial growth.
Even within a single day, the syringe filter clogs after repeated use. If it feels like you need to press harder to dispense, replace the syringe filter.
Each syringe filter typically clogs after 200–500 mL of liquid dispensed (20–50 dispenses of 10 mL).
Indications of clogged syringe filter
A squeaking sound may indicate a clogged syringe filter. Replace the syringe filter if squeaking occurs to maintain accurate and fluent dispensing.
