BactoBox® uses Impedance Flow Cytometer (IFC). That sounds very technical, but let's look at each words one by one.
BactoBox explainer. For best experience turn on sound .
Impedance
Impedance is the cell’s resistance to electrical current. All cells have lipid membranes, and lipid membranes do not conduct electricity well. Because of this, the membrane acts as a barrier that changes how electrical current flows between the electrodes when a cell passes through the measurement area.
At low electrical measurement frequencies current mostly goes around the bacterium because the membrane acts like an insulator
At high electrical measurement frequencies current can partly pass through the cell membrane and probe the inside of the bacterium. The cytoplasm of cells is usually rich in ions and is good at conducting electricity.
BactoBox® measurement principle. The membrane and cytoplasm of cells creates a distinct electrical signature.
Flow
Flow means movement of liquid. This is what we refer to when liquid is passing through the flow cell. BactoBox® can determine the flow rate by measuring how fast an object travels between electrode sets.
Cytometry
Cyto means cell. Metry means "to measure". So cytometry is any method used to measure properties of cells such as number of cells, size, shape, and electrical properties. BactoBox® counts cells by counting objects with distinct membrane-like features when these are moving past the electrodes.
In a nutshell
Now that we know what each word means let's try to put it in into practical relevance. BactoBox® uses IFC to determine the concentration of bacteria. This is similar to Fluorescence Flow Cytometry (FFC) in that objects traverse the detector element in a single file. Unlike FFC, which uses lasers, BactoBox® uses an electric field as its detector element. The lipid membrane of bacteria and conductive cytoplasm provides a distinct signature that enables specific detection of bacteria without the need for staining.
In other words, BactoBox® uses IFC to enable easy, fast, precise, and specific enumeration of cells.
