> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/more/how-to-guides/deep-clean-your-setup.md).
# Deep clean your setup
Clear limescale and biofilm from the flow path with a deep clean vial. The weekly deep clean takes 15 minutes.
Limescale and biofilm build up in your flow cell over time. Its narrowest channel is 10 × 25 µm, so even a light build-up affects your measurements.
## When to deep clean
Deep clean your BactoBox® in these cases:
* **Every week you use your BactoBox®.** Use the 15-minute option.
* Before your first measurement after more than a week without use.
* To recover a flow cell that failed the QC test. Try the 15-minute option first, and the 10-hour option if the problem remains.
## Step by step
{% stepper %}
{% step %}
#### Move the tubing kit to a deep clean vial
Place a [deep clean vial](/item-register/consumables/deep-clean-vial.md) in the vial rack and move the tubing kit to it.
{% endstep %}
{% step %}
#### Start the *Deep clean* program
Press and hold Measure for 2 seconds to open the *Programs* menu. Use the navigation buttons (OK←∨∧) to select *Deep clean*, and press OK.
{% endstep %}
{% step %}
#### Select the duration
Select the duration, usually 15 minutes, and press OK to start.
{% endstep %}
{% step %}
#### Close and discard the deep clean vial
When the program is done, the deep clean solution is back in the vial. Close the vial with its screw cap and discard it as biological waste. It is for single use only.
{% endstep %}
{% step %}
#### Clean with a disinfection vial
Run a [Clean](/more/how-to-guides/clean-your-setup.md) with a [disinfection vial](/item-register/consumables/disinfection-vial.md). Its 70 % denatured alcohol flushes out any deep clean solution left in the flow path.
{% endstep %}
{% step %}
#### Run a QC test
Run a [QC test](/maintenance/qc-test.md) to check that your BactoBox® measures correctly.
{% endstep %}
{% endstepper %}
---
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