# Bioprocess

A bioprocess uses living [cells](broken://pages/HyRrklD1byQRCoLKvOCE#cell) to produce a [Bioproduct](/mpd/encyclopedia/bioproduct.md).

In the context of BactoBox®, we focus on [microbial](broken://pages/HyRrklD1byQRCoLKvOCE#microbial) bioprocesses. That is, bioprocesses with cells in the micrometer range.

A bioprocess is the result of [Bioproduct](/mpd/encyclopedia/bioproduct.md).

## Upstream and downstream

A bioprocess has two subprocesses: Upstream and downstream.

<img src="/files/QeTSrVKp1UPfLyGOKZ5J" alt="Bioprocess divided into upstream and downstream." class="gitbook-drawing">

## Upstream bioprocess

In practical terms, an upstream bioprocess is everything that goes on inside a [vessel](broken://pages/HyRrklD1byQRCoLKvOCE#vessel-or-reaction-vessel) (e.g., inside a bioreactor).

The key steps are:

* 🌱 You seed (inoculate) the [vessel](broken://pages/HyRrklD1byQRCoLKvOCE#vessel-or-reaction-vessel) (e.g., bioreactor) with your [seed culture](broken://pages/HyRrklD1byQRCoLKvOCE#seed-culture-or-starter-culture-or-inoculum).
* 🌿 You wait for your [culture](broken://pages/HyRrklD1byQRCoLKvOCE#culture-or-cell-culture) to grow inside the controlled environment of the vessel.
* 🌽 You harvest the culture for further processing downstream.

The goal of the upstream bioprocess is to get a high-quality culture for harvest.

<img src="/files/bSAWh4s9OB2VZBAr7zoQ" alt="" class="gitbook-drawing">


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