> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/encyclopedia/get-starter-cultures/from-crm-product/get-things-ready.md).
# Get things ready
You will need these items for the protocol.
## Hardware
* [BactoBox® setup](/item-register/bactobox-r/bactobox-r.md) including a [flow cell](/item-register/consumables/flow-cell.md).
* HW: [7.6a](/item-register/bactobox-r/bactobox-r-hardware-changelog-retired.md)
* SW: ≥ v2026.02a
* Vortex mixer or alternatively a bead beater like [bead genie](https://www.scientificindustries.com/products/bead-genie%E2%84%A2?srsltid=AfmBOopRmRHhvdvEtPAo7snGZNBxssDgbZpMhqlaAFGGuBxu2WSzUJrF).
* Rack for holding 5 and 15 mL centrifuge vials.
* Sterile 1 µL Inoculation loops. Preferably made of polystyrene (PS). See below hint.
* P200 or P100 pipette.
* Sterile pipette tips.
{% hint style="success" %}
#### Use polystyrene (PS) inoculation loops
After picking a suitable colony, you will snap the inoculation loop in the capture vial. Choose [polystyrene (PS)](https://www.sarstedt.com/en/products/laboratory/microbiology/accessories/product/86.1567.050/) because they snap easier than more "bendable" materials like polypropylene.
{% endhint %}
## Reagents
* 1 × [Lyfo diskTM](https://www.microbiologics.com/item-type/Product/product-format/LYFO-DISK).
* 1 × agar plate. Preferably same agar type as the liquid growth medium.
* 1 × shake flask with growth medium. E.g. 250 mL flask with max 50 mL growth medium.
* 2 × capture vials: [5 mL centrifuge vial with screw cap](https://www.eppendorf.com/dk-en/Products/Lab-Consumables/Lab-Tubes/EppendorfTubes-50mL-p-PF-156668) containing 4 mL cultivation medium and 1 mL 3 mm glass beads. See below hint box for more information.
* [Dilution vials](/item-register/individual-items/dilution-vial.md) either ready-to-use or freshly prepared using the [dilution dispenser](/item-register/accessories/dilution-dispenser.md).
* [Disinfection vials](/item-register/consumables/disinfection-vial.md).
With these things in hand, you are ready to [Rehydrate and streak](/mpd/encyclopedia/get-starter-cultures/from-crm-product/rehydrate-and-streak.md).
---
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