Where you end up: a full growth curve, plotted live in Access.
1
Open Access
Connect your BactoBox® to your computer and open Access in your browser. The familiar BactoBox® buttons move onto your screen.
Plug in your BactoBox® and open Access.
2
Create a measurement group
Start a growth curve measurement group. Every sample lands in the same place, ready to plot.
One measurement group collects every sample.
3
Measure and annotate each sample
Set the dilution factor and a label, then click Measure to run the measurement. Access works out the concentration in your flask. Use the same label for every sample in the series, for example E_coli_growth, then click that label above the chart to watch your curve build.
The label is what ties the curve together
The label is not a free-form note. Access plots every measurement that shares a label as one growth curve, and places each point on the time axis by its measurement time. So give every sample in the series the same label. If you change the label between samples, you get scattered single points instead of one curve.
Annotate, measure, and the curve builds point by point.
4
Watch it reach stationary
Keep sampling, with a Clean after each measurement. Continue to see the onset of stationary phase.
The curve flattens as the culture reaches stationary phase.
Do it yourself
That is the whole loop. The full guide walks you through sample prep, dilution schemes, and the medium check, step by step.
