> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/workflows.md). # Workflows - [Bioprocess workflows](https://help.sbtinstruments.com/mpd/workflows/bioprocess-workflows.md) - [Optimize your bioprocess](https://help.sbtinstruments.com/mpd/workflows/bioprocess-workflows/optimize-your-bioprocess.md) - [Understand your bioprocess](https://help.sbtinstruments.com/mpd/workflows/bioprocess-workflows/understand-your-bioprocess.md) - [Get starter culture](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures.md): MPD-5 - [From cryo stock](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-cryo-stock.md) - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-cryo-stock/get-things-ready.md) - [Inoculate culture](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-cryo-stock/inoculate-culture.md) - [Determine initial concentration](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-cryo-stock/determine-initial-concentration.md) - [Determine concentration after incubation](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-cryo-stock/determine-concentration-after-incubation.md) - [From agar plate colony](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-agar-plate-colony.md): Get an active culture using a fresh colony from an agar plate. - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-agar-plate-colony/get-things-ready.md) - [Collect and disperse](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-agar-plate-colony/collect-and-disperse.md) - [Determine concentration](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-agar-plate-colony/determine-concentration.md) - [Inoculate liquid culture](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-agar-plate-colony/inoculate-liquid-culture.md) - [Determine concentration after incubation](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-agar-plate-colony/determine-concentration-after-incubation.md) - [From liquid culture](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-liquid-culture.md) - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-liquid-culture/get-things-ready.md) - [Determine concentration of inoculum](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-liquid-culture/determine-concentration-of-inoculum.md) - [Inoculate culture](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-liquid-culture/inoculate-culture.md) - [Determine initial concentration](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-liquid-culture/determine-initial-concentration.md) - [Determine concentration after incubation](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-liquid-culture/determine-concentration-after-incubation.md) - [From CRM product](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-crm-product.md) - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-crm-product/get-things-ready.md) - [Rehydrate and streak](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-crm-product/rehydrate-and-streak.md) - [Collect and disperse](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-crm-product/collect-and-disperse.md) - [Determine concentration](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-crm-product/determine-concentration.md) - [Inoculate liquid culture](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-crm-product/inoculate-liquid-culture.md) - [Determine concentration after incubation](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-crm-product/determine-concentration-after-incubation.md) - [Optimize starter culture](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size.md): MPD-6. Get a high-quality overnight culture after the desired incubation time. - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/get-things-ready.md) - [Prepare flasks and media](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/prepare-flasks-and-media.md) - [Create measurement group](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/create-measurement-group.md) - [Measure on cryo stock](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/measure-on-cryo-stock.md) - [Prepare dilution series](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/prepare-dilution-series.md) - [Select relevant cultures](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/select-relevant-cultures.md) - [Check lowest culture](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/check-lowest-culture.md) - [Check remaining cultures](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/check-remaining-cultures.md) - [Choose best seeding ratio](https://help.sbtinstruments.com/mpd/workflows/identify-optimal-inoculum-size/choose-best-seeding-ratio.md) - [Track growth curve](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve.md): MPD-7. Visualize growth curves in real-time with BactoBox®. - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/get-things-ready.md) - [Create measurement group](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/create-measurement-group.md) - [Check growth medium](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/check-growth-medium.md) - [Measure starter culture](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/measure-starter-culture.md) - [Inoculate](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/inoculate.md) - [1:100 dilution after inoculation](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/1-100-dilution-post-inoculation.md) - [1:100 dilutions](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/1-100-dilutions.md) - [1:1 000 dilutions](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/1-1-000-dilutions.md) - [1:10 000 dilutions](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/1-10-000-dilutions.md) - [Conclude growth curve](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/conclude-growth-curve.md) - [Screen growth media](https://help.sbtinstruments.com/mpd/workflows/best-medium.md): MPD-3. Use BactoBox® direct cell counts to screen growth media for their ability to support high cell concentrations. - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/best-medium/get-things-ready.md) - [Create measurement group](https://help.sbtinstruments.com/mpd/workflows/best-medium/create-measurement-group.md) - [Morning measurement](https://help.sbtinstruments.com/mpd/workflows/best-medium/check-remaining-cultures.md) - [Noon measurement](https://help.sbtinstruments.com/mpd/workflows/best-medium/check-remaining-cultures-1.md) - [Afternoon measurement](https://help.sbtinstruments.com/mpd/workflows/best-medium/check-remaining-cultures-2.md) - [Identify best medium](https://help.sbtinstruments.com/mpd/workflows/best-medium/identify-best-medium.md) - [Inspect data critically](https://help.sbtinstruments.com/mpd/workflows/best-medium/inspect-data-critically.md) - [Summary](https://help.sbtinstruments.com/mpd/workflows/best-medium/summary.md) - [End-of-fermentation cross-check](https://help.sbtinstruments.com/mpd/workflows/cross-check.md): Run an endpoint BactoBox® vs CFU comparison to check if your bioprocess has further potential. - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/cross-check/get-things-ready.md) - [Create measurement group](https://help.sbtinstruments.com/mpd/workflows/cross-check/create-measurement-group.md) - [Retrieve culture sample](https://help.sbtinstruments.com/mpd/workflows/cross-check/retrieve-culture-sample.md) - [BactoBox measurements](https://help.sbtinstruments.com/mpd/workflows/cross-check/bactobox-measurements.md) - [Calculate dilutions](https://help.sbtinstruments.com/mpd/workflows/cross-check/calculations-for-plating.md) - [Plate dilutions](https://help.sbtinstruments.com/mpd/workflows/cross-check/plate-dilutions.md) - [Count colonies](https://help.sbtinstruments.com/mpd/workflows/cross-check/check-remaining-cultures.md) - [Cross-check data](https://help.sbtinstruments.com/mpd/workflows/cross-check/cross-check-data.md) - [Summary](https://help.sbtinstruments.com/mpd/workflows/cross-check/summary.md) - [Identify harvest time](https://help.sbtinstruments.com/mpd/workflows/mpd1.md): MPD-1. Determine best harvest time for highest cell concentration in batch cultivation. - [Plan experimental timeline](https://help.sbtinstruments.com/mpd/workflows/mpd1/plan-experimental-timeline.md) - [Get things ready](https://help.sbtinstruments.com/mpd/workflows/mpd1/get-things-ready.md) - [Create measurement group](https://help.sbtinstruments.com/mpd/workflows/mpd1/create-measurement-group.md) - [Track deceleration phase](https://help.sbtinstruments.com/mpd/workflows/mpd1/track-deceleration-phase.md) - [Track hourly](https://help.sbtinstruments.com/mpd/workflows/mpd1/track-hourly.md) - [Conclude engineering run](https://help.sbtinstruments.com/mpd/workflows/mpd1/conclude-engineering-run.md) - [Inspect data for harvest time](https://help.sbtinstruments.com/mpd/workflows/mpd1/inspect-data-for-harvest-time.md) - [Summary](https://help.sbtinstruments.com/mpd/workflows/mpd1/summary.md) - [Optional: rigorous approach](https://help.sbtinstruments.com/mpd/workflows/mpd1/optional-rigorous-approach.md) - [Plate smarter](https://help.sbtinstruments.com/mpd/workflows/plate-smarter.md): MPD-8: Use BactoBox® to find the right dilution for plate counts --- # Agent Instructions This documentation is published with GitBook. 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