Afternoon measurement

We allow the cultures to incubate additional ~4 hours for the afternoon measurement.

The below video demonstrates the sample workup. It is the exactly the same as in the morning measurement.

1

Retrieve a sample

Use a serological pipette to sample 1 mL culture. Transfer to a 5 mL centrifuge tube. Note down the sampling time.

2

Disaggregate cell clumps

Vortex the vial 1 minute at maximum speed to disaggregate cell clumps. See Break up clumps and chains if your bacterium needs more aggressive disaggregation than this.

3

Dilute 1:100

Transfer 101 µL of your sample to 10 mL of diluent. Vortex 10 seconds at maximum speed.

4

Dilute 1:10 000

Transfer 101 µL of the diluted sample to 10 mL of diluent. Vortex 10 seconds at maximum speed.

5

Measure the diluted sample on BactoBox®

Transfer the tubing kit to the 1:10 000 vial.

Press Measure in Access to start a new measurement.

• Dilution: 10 000

• Label: Growth_medium_type . For example LB or TSB.

After completing the measurement, Access will automatically calculate the cell concentration in the culture in the cellsCells/mL × Dil.

6

Check remaining cultures

Repeat above steps with 1:10 000 dilutions of the remaining growth media.

Summary

You have now determined the cell concentration in the different growth media cultures. Proceed to the next step for the calculations to Identify best medium.