Get things ready

You will need these items for the experiment.

Hardware

• BactoBox® setup including a flow cell with at least 10 remaining measurements.

• Vortex mixer.

• Rack for holding 5 and 15 mL centrifuge vials.

• P1000 and P200 pipette.

• Sterile pipette tips.

• Serological pipettes. Preferably 2 mL version.

• Serological pipet controller.

• Marker

Reagents

• Cultures of the same species in different growth media

  • For example E. coli in 50 mL growth medium in a shake flask with 250 mL total volume.

  • The cultures should be in stationary or decline growth phase.

  • Different growth media should be used. Follow the suppliers recommendations on how to prepare the growth medium.

• Dilution vials either ready-to-use or freshly prepared using the dilution dispenser.

• Disinfection vials.

Summary

With these things in hand, you are ready to Create measurement group.