> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/workflows/best-medium/check-remaining-cultures-2.md).
# Afternoon measurement
We allow the cultures to incubate additional \~4 hours for the afternoon measurement.
The below video demonstrates the sample workup. It is the exactly the same as in the morning measurement.
{% stepper %}
{% step %}
#### Retrieve a sample
Use a serological pipette to sample 1 mL culture. Transfer to a 5 mL centrifuge tube. Note down the sampling time.
{% endstep %}
{% step %}
**Disaggregate cell clumps**
Vortex the vial 1 minute at maximum speed to disaggregate cell clumps. See [Break up clumps and chains](https://help.sbtinstruments.com/custom/advanced-sample-preparation/break-up-clumps-and-chains) if your bacterium needs more aggressive disaggregation than this.
{% endstep %}
{% step %}
**Dilute 1:100**
Transfer 101 µL of your sample to 10 mL of diluent. Vortex 10 seconds at maximum speed.
{% endstep %}
{% step %}
**Dilute 1:10 000**
Transfer 101 µL of the diluted sample to 10 mL of diluent. Vortex 10 seconds at maximum speed.
{% endstep %}
{% step %}
**Measure the diluted sample on BactoBox®**
Transfer the tubing kit to the 1:10 000 vial.
Press Measure in Access to start a new measurement.
* Dilution: 10 000
* Label: Growth\_medium\_type . For example LB or TSB.
After completing the measurement, Access will automatically calculate the cell concentration in the culture in the cellsCells/mL × Dil.
{% endstep %}
{% step %}
**Check remaining cultures**
Repeat above steps with 1:10 000 dilutions of the remaining growth media.
{% endstep %}
{% endstepper %}
## Summary
You have now determined the cell concentration in the different growth media cultures.\
Proceed to the next step for the calculations to [Identify best medium](/mpd/workflows/best-medium/identify-best-medium.md).
---
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