> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/workflows/best-medium/get-things-ready.md).

# Get things ready

You will need these items for the experiment.

<figure><img src="/files/qWfRFiOiejPyOwwJH1HO" alt=""><figcaption></figcaption></figure>

## Hardware

* [BactoBox® setup](https://help.sbtinstruments.com/items/bactobox-r#bactobox-r-setup) including a [flow cell](https://help.sbtinstruments.com/items/consumables/flow-cell) with at least 10 remaining measurements.
  * HW: [7.6a](/item-register/bactobox-r/bactobox-r-hardware-changelog.md)
  * SW: [v2026.02a](https://help.sbtinstruments.com/software/)
* Vortex mixer.
* Rack for holding 5 and 15 mL centrifuge vials.
* P1000 and P200 pipette.
* Sterile pipette tips.
* Serological pipettes. Preferably 2 mL version.
* Serological pipet controller.
* Marker

## Reagents

* Cultures of the same species in different growth media
  * For example *E. coli* in 50 mL growth medium in a shake flask with 250 mL total volume.
  * The cultures should be in stationary or decline [growth phase](/mpd/cell-growth/growth-phases.md).
  * Different growth media should be used. Follow the suppliers recommendations on how to prepare the growth medium.
* [Dilution vials](https://help.sbtinstruments.com/items/vials-flasks-and-liquids/dilution-vial) either ready-to-use or freshly prepared using the [dilution dispenser](https://help.sbtinstruments.com/items/accessories/dilution-dispenser).
* [Disinfection vials](https://help.sbtinstruments.com/items/vials-flasks-and-liquids/disinfection-vial).

## Summary

With these things in hand, you are ready to [Create measurement group](/mpd/workflows/best-medium/create-measurement-group.md).


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