End-of-fermentation cross-check
Run an endpoint BactoBox® vs CFU comparison to check if your bioprocess has further potential.
Ever wondered if your fermentation process has more potential? Getting maximal CFU/mL is crucial for vaccines, probiotics, bio-stimulants, and live biotherapeutic products. A simple crosscheck at end-of-fermentation (EoF) may unveil critical insights that unlocks higher CFU/mL.
Cross-check BactoBox® and plate count results
The importance of harvest time is illustrated in the below P. fluorescens example.
In the cyan window, the cell concentration is practically identical to the concentration of culturable (cells ~ CFU). Harvesting later could potentially increase the CFU/mL of the final product.
In the grey window the cell concentration is significantly higher than the proportion of culturable cells (cells > CFU). Harvesting earlier could potentially increase the CFU/mL of the final product.
Overview
In this step by step guide we will demonstrate how to crosscheck BactoBox® and CFU results. You will need a fresh EoF culture sample. This should preferably be from a bioreactor, but you can also simulate the process with a simple shake flask culture of E. coli.
The overall steps in the workflow are given below.
Summary
With this introduction you are now ready to check if your bioprocess has higher potential. Next step is Get things ready.
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