> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/workflows/cross-check/get-things-ready.md).

# Get things ready

You will need these items for the experiment.

<figure><img src="/files/VhFrDE4rwBn6NmDkaC4t" alt=""><figcaption></figcaption></figure>

## Hardware

* [BactoBox® setup](https://help.sbtinstruments.com/items/bactobox-r#bactobox-r-setup) including a [flow cell](https://help.sbtinstruments.com/items/consumables/flow-cell) with at least 10 remaining measurements.
  * HW: [7.6a](/item-register/bactobox-r/bactobox-r-hardware-changelog.md)
  * SW: [v2026.02a](https://help.sbtinstruments.com/software/)
* Vortex mixer.
* Rack for holding 5 and 15 mL centrifuge vials.
* Cold rack for holding 5 and 15 mL centrifuge vials, e.g. [ice rack](https://www.sigmaaldrich.com/DK/en/product/sigma/baf189050050), [chillblock](https://www.sigmaaldrich.com/DK/en/product/sigma/cls432061), or [mini cooler](https://eu.raptorsupplies.com/pd/globe-scientific/454005). You can also use a simple expanded polystyrene with a mix of water and ice (or a cooling element).
* P1000 and P200 pipette.
* Sterile pipette tips.
* Serological pipettes. Preferably 5 mL version.
* Serological pipet controller.
* Marker

## Reagents

* EoF[^1] culture sample. Preferably from a bioreactor. Alternatively use an *E. coli* culture presumed to be in [stationary growth phase](/mpd/cell-growth/growth-phases.md). See [Get starter culture](/mpd/workflows/get-starter-cultures.md) for more information.
* [SBT dilution vial](/item-register/vials-flasks-and-liquids/dilution-vial.md) or [DIY dilution vial](/item-register/accessories/dilution-dispenser.md).
* [Disinfection vials](https://help.sbtinstruments.com/items/vials-flasks-and-liquids/disinfection-vial).
* 9 mL aliquots of [sterile, buffered peptone water](https://www.neogen.com/en/categories/microbiology/mini-flip-top-buffered-peptone-water-broth/?srsltid=AfmBOooH6h4Y-fsb54jpinvtYZJ0CKBV_FyHDX_rM4C2rxqrbtRdJpYS) (or similar).
* Consumables for plate counts, e.g. [compact dry TC](https://www.compact-dry.me/) plates.

## Summary

With these things in hand, you are ready to next step: [Create measurement group](/mpd/workflows/cross-check/create-measurement-group.md).

[^1]: End-of-Fermentation


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