Get things ready

You will need these items for the experiment.

Hardware

• BactoBox® setup including a flow cell with at least 10 remaining measurements.

  • HW: 7.6a

  • SW: v2026.02a

• Vortex mixer.

• Rack for holding 5 and 15 mL centrifuge vials.

• Cold rack for holding 5 and 15 mL centrifuge vials, e.g. ice rack, chillblock, or mini cooler. You can also use a simple expanded polystyrene with a mix of water and ice (or a cooling element).

• P1000 and P200 pipette.

• Sterile pipette tips.

• Serological pipettes. Preferably 5 mL version.

• Serological pipet controller.

• Marker

Reagents

• EoF culture sample. Preferably from a bioreactor. Alternatively use an E. coli culture presumed to be in stationary growth phase. See Get starter culture for more information.

• SBT dilution vial or DIY dilution vial.

• Disinfection vials.

• 9 mL aliquots of sterile, buffered peptone water (or similar).

• Consumables for plate counts, e.g. compact dry TC plates.

Summary

With these things in hand, you are ready to next step: Create measurement group.