# Collect and disperse

A fresh colony is collected using an inoculation loop and the cells are dispersed in a 5 mL centrifuge vial. We refer to this as the capture vial.

{% hint style="success" icon="dice-one" %}

#### Collect a single, well-isolated, discrete colony

To ensure a single genetic origin (monoclonal), it is best to collect a well-isolated, discrete colony. Avoid fused colonies, as they may contain different clones. For very small colonies, it may be necessary to gather several to achieve a BactoBox® result within the desired range.
{% endhint %}

The video shows all the collect-and-disperse steps.

{% stepper %}
{% step %}
**Collect a colony**

Use a 1 µm inoculation loop to collect a single, fresh colony. See the above info field for details
{% endstep %}

{% step %}
**Transfer colony to capture vial**

Insert the tip of the inoculation loop into the capture vial containing 3 mm glass beads. Press the screw cap down on the rod while bending the loop up and down until the rod snaps. Tighten the cap securely.
{% endstep %}

{% step %}
**Disperse cells**

Use [bead-genie](https://www.scientificindustries.com/products/bead-genie%E2%84%A2?srsltid=AfmBOopeCmTHw8Fo4wj9wkggx4sjCx21NFo6YwXjXQwrP5Ks5Rf3_702) for 1 minute at max speed to disperse cells. If you don't have a bead-beater beater, vortex the capture vial 1 min at max speed.
{% endstep %}
{% endstepper %}

## Summary

A suitable colony is collected, and the dispersed cells in the capture vial are now ready for BactoBox® analysis. Next step is to [determine the cell concentration](/mpd/workflows/get-starter-cultures/from-agar-plate-colony/determine-concentration.md).


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