Determine concentration

The dispersed cells in the capture vial will be used to inoculate a liquid culture at a well-defined seeding concentration. To know which volume to add, first determine the cell concentration and then use the subcultivation calculator to determine the volume. The steps are demonstrated below.

1

Prepare a 1:201 dilution

Transfer 50 µL of your sample to 10 mL of diluent. Vortex 10 seconds at maximum speed.

2

Measure on the diluted sample

Transfer the tubing kit to the diluted sample. Press Measure and wait for the result to appear on the BactoBox® screen.

3

Calculate cell concentration in capture vial

Multiply the cells/mL with the dilution factor. In this case, it is 201

Summary

Now you know the cell concentration in the capture vial. Proceed to Inoculate liquid culture to calculate the volume of capture vial suspension needed to inoculate growth medium in the shake flask.