From CRM product

Skill level	Time to complete	Hands-on time	HW requirement
Intermediate	2 days (E. coli)	4 hours	7.6a

Certified reference materials are available from e.g. Microbiologics, ATCC, bioMérieux, Merck, Zeptometrix, and Thermo Scientific. Some formats require different preparations:

• Agar stabs: Need disaggregation.

• Disk-types: Require rehydration.

• Glycerol stocks: Must be thawed.

In this step by step guide we provide an example of how to work with the Microbiologics lyfo disk^TM. In principle the disks are compatible with the workflow From agar plate colony and are also compatible with the workflow From liquid culture.

Here we use the workflow From agar plate colony in combination with the lyfo disk^TM. First the disk is rehydrated and the cell-containing rehydrate is used to streak an agar plate. Once single, fresh colonies are available they are used to inoculate growth medium at a well-defined concentration.

The step by step guide is divided in the following sections.

1. Get things ready

2. Rehydrate and streak

3. Collect and disperse

4. Determine concentration

5. Inoculate liquid culture

6. Determine concentration after incubation

See Microbiologics lyfo disk^TM documentation for full details

We use an adapted version of the lyfo disk^TM rehydration protocol. For full description please inspect the original supporting material.

Proceed to Get things ready for a check list of items necessary for the workflow.