# From CRM product

| Skill level  | Time to complete                                           | Hands-on time                                   | Requirements                                                                                                                                                                                                 |
| ------------ | ---------------------------------------------------------- | ----------------------------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ |
| Intermediate | <i class="fa-hourglass">:hourglass:</i> 2 days (*E. coli*) | <i class="fa-stopwatch">:stopwatch:</i> 4 hours | <i class="fa-hard-drive">:hard-drive:</i> [7.6a](/encyclopedia/item-register/bactobox-r/bactobox-r-hardware-changelog.md) <i class="fa-floppy-disk">:floppy-disk:</i> [v2026.02a](/encyclopedia/software.md) |

Certified reference materials are available from e.g. [Microbiologics](https://www.microbiologics.com/item-type/Product/product-format/Epower), [ATCC](https://www.atcc.org/products/qc6-mini), [bioMérieux](https://biotek.com.mk/product/bioball/), [Merck](https://www.sigmaaldrich.com/DK/en/campaigns/vitroids-and-lenticule-discs?srsltid=AfmBOophpxaxYomocMiekPqjIwgOoQEeebzBEdrsLsVrRk2JCjnYVgt9), [Zeptometrix](https://www.zeptometrix.com/dk/en/analytical-reference-materials/microbiology/food-and-agriculture?product_type%5B%5D=Microbiology+Standards\&ipp=12), and [Thermo Scientific](https://documents.thermofisher.com/TFS-Assets/MBD/brochures/Culti-Loops%20Brochure.pdf). Some formats require different preparations:

* **Agar stabs:** Need disaggregation.
* **Disk-types:** Require rehydration.
* **Glycerol stocks:** Must be thawed.

In this step by step guide we provide an example of how to work with the [Microbiologics](https://www.microbiologics.com/item-type/Product/product-format/Epower) [lyfo disk<sup>TM</sup>](https://www.microbiologics.com/item-type/Product/product-format/LYFO-DISK). In principle the disks are compatible with the workflow [From agar plate colony](/mpd/workflows/get-starter-cultures/from-agar-plate-colony.md) and are also compatible with the workflow [From liquid culture](/mpd/workflows/get-starter-cultures/from-liquid-culture.md).

Here we use the workflow [From agar plate colony](/mpd/workflows/get-starter-cultures/from-agar-plate-colony.md) in combination with the [lyfo disk<sup>TM</sup>](https://www.microbiologics.com/item-type/Product/product-format/LYFO-DISK). First the disk is rehydrated and the cell-containing rehydrate is used to streak an agar plate. Once single, fresh colonies are available they are used to inoculate growth medium at a well-defined concentration.

The step by step guide is divided in the following sections.

1. [Get things ready](/mpd/workflows/get-starter-cultures/from-crm-product/get-things-ready.md)
2. [Rehydrate and streak](/mpd/workflows/get-starter-cultures/from-crm-product/rehydrate-and-streak.md)
3. [Collect and disperse](/mpd/workflows/get-starter-cultures/from-crm-product/collect-and-disperse.md)
4. [Determine concentration](/mpd/workflows/get-starter-cultures/from-crm-product/determine-concentration.md)
5. [Inoculate liquid culture](/mpd/workflows/get-starter-cultures/from-crm-product/inoculate-liquid-culture.md)
6. [Determine concentration after incubation](/mpd/workflows/get-starter-cultures/from-crm-product/determine-concentration-after-incubation.md)

{% hint style="info" %}

#### See [Microbiologics](https://www.microbiologics.com/item-type/Product/product-format/Epower) [lyfo disk<sup>TM</sup>](https://www.microbiologics.com/item-type/Product/product-format/LYFO-DISK) documentation for full details

We use an adapted version of the [lyfo disk<sup>TM</sup>](https://www.microbiologics.com/item-type/Product/product-format/LYFO-DISK) rehydration protocol. For full description please inspect the original [supporting material](https://www.microbiologics.com/item-type/Product/product-format/LYFO-DISK).
{% endhint %}

Proceed to [Get things ready](/mpd/workflows/get-starter-cultures/from-crm-product/get-things-ready.md) for a check list of items necessary for the workflow.


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