Collect and disperse

A fresh colony is collected using an inoculation loop and the cells are dispersed in a 5 mL centrifuge vial. We refer to this as the capture vial.

Collect a single, well-isolated, discrete colony

To ensure a monoclonal origin, it is best to collect a well-isolated, discrete colony. Avoid fused colonies, as they may contain different clones. For very small colonies, it may be necessary to gather several to achieve a BactoBox® result within the desired range.

The video shows the collect-and-disperse steps.

1

Collect a colony

Use a 1 µm inoculation loop to collect a single, fresh colony. See the above info field for details

2

Transfer colony to capture vial

Insert the tip of the inoculation loop into the capture vial containing 3 mm glass beads. Press the screw cap down on the rod while bending the loop up and down until the rod snaps. Tighten the cap securely.

3

Disperse cells

Use bead-genie for 1 minute at max speed to disperse cells. If you don't have a bead-beater beater, vortex the capture vial 1 min at max speed.

Summary

A suitable colony has been collected, and the dispersed cells in the capture vial are now ready for BactoBox® analysis. Next step is to Determine concentration.