# Get things ready

You will need these items for the protocol.

<figure><img src="/files/VhFrDE4rwBn6NmDkaC4t" alt=""><figcaption></figcaption></figure>

## Hardware

* [BactoBox® setup](https://help.sbtinstruments.com/encyclopedia/item-register/bactobox-r#bactobox-r-setup) including a [flow cell](https://help.sbtinstruments.com/encyclopedia/item-register/consumables/flow-cell).
  * HW: [7.6a](/encyclopedia/item-register/bactobox-r/bactobox-r-hardware-changelog.md)&#x20;
  * SW: [v2026.02a](/encyclopedia/software.md)
* Vortex mixer or alternatively a bead beater like [bead genie](https://www.scientificindustries.com/products/bead-genie%E2%84%A2?srsltid=AfmBOopRmRHhvdvEtPAo7snGZNBxssDgbZpMhqlaAFGGuBxu2WSzUJrF).
* Rack for holding 5 and 15 mL centrifuge vials.
* Sterile 1 µL Inoculation loops. Preferably made of polystyrene (PS). See below hint.
* P200 or P100 pipette.
* Sterile pipette tips.

{% hint style="success" %}

#### Use polystyrene (PS) inoculation loops

After picking a suitable colony, you will snap the inoculation loop in the capture vial. Choose [polystyrene (PS)](https://www.sarstedt.com/en/products/laboratory/microbiology/accessories/product/86.1567.050/) because they snap easier than more "bendable" materials like polypropylene.
{% endhint %}

## Reagents

* 1 × [Lyfo disk<sup>TM</sup>](https://www.microbiologics.com/item-type/Product/product-format/LYFO-DISK).
* 1 × agar plate. Preferably same agar type as the liquid growth medium.
* 1 × shake flask with growth medium. E.g. 250 mL flask with max 50 mL growth medium.
* 2 × capture vials: [5 mL centrifuge vial with screw cap](https://www.eppendorf.com/dk-en/Products/Lab-Consumables/Lab-Tubes/EppendorfTubes-50mL-p-PF-156668) containing 4 mL cultivation medium and 1 mL 3 mm glass beads. See below hint box for more information.
* [Dilution vials](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/dilution-vial) either ready-to-use or freshly prepared using the [dilution dispenser](https://help.sbtinstruments.com/encyclopedia/item-register/accessories/dilution-dispenser).
* [Disinfection vials](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/disinfection-vial).

With these things in hand, you are ready to [Rehydrate and streak](/mpd/workflows/get-starter-cultures/from-crm-product/rehydrate-and-streak.md).


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