Get things ready

You will need these items for the protocol.

Hardware

• BactoBox® setup including a flow cell.

• Vortex mixer or alternatively a bead beater like bead genie.

• Rack for holding 5 and 15 mL centrifuge vials.

• Sterile 1 µL Inoculation loops. Preferably made of polystyrene (PS). See below hint.

• P200 or P100 pipette.

• Sterile pipette tips.

Use polystyrene (PS) inoculation loops

After picking a suitable colony, you will snap the inoculation loop in the capture vial. Choose polystyrene (PS) because they snap easier than more "bendable" materials like polypropylene.

Reagents

• 1 × lyfo disk^TM.

• 1 × agar plate. Preferably same agar type as the liquid growth medium.

• 1 × shake flask with growth medium. E.g. 250 mL flask with max 50 mL growth medium.

• 2 × capture vials: 5 mL centrifuge vial with screw cap containing 4 mL cultivation medium and 1 mL 3 mm glass beads. See below hint box for more information.

• Dilution vials either ready-to-use or freshly prepared using the dilution dispenser.

• Disinfection vials.

With these things in hand, you are ready to Rehydrate and streak.