Rehydrate and streak

The first goal to obtain a streaked agar plate of single, well-isolated colonies. The lyfo disk^TM is used as a starting material.

See Microbiologics lyfo disk^TM documentation for full details

We use an adapted version of the lyfo disk^TM rehydration protocol. For full description please inspect the supporting material provided by Microbiologics.

Step by step

1

Transfer disk to capture tube

Use aseptic technique to transfer a LYFO DISK to a preheated (37 °C) 5 mL capture tube containing glass beads.

2

Dissolve disk and disperse cells

Rehydrate the disk by bead-beating, manual shaking or vortexing for 1 min at max speed (or until the suspension is homogenous)

3

Streak agar plate

Dip a sterile 1 µL inoculate loop into the suspension and streak to obtain single, well-resolved colonies.

4

Incubate

Incubate at optimal growth conditions until colonies appear (typically overnight). Avoid over-incubation as this may lead to presence of dead cells.

Summary

The lyfo disk^TM is rehydrated and an agar plate is streaked to get single colonies. Next step is to Collect and disperse.