snowflakeFrom cryo stock

Skill level
Time to complete
Hands-on time
HW requirement

Intermediate

hourglass 16 hours (E. coli)

stopwatch 3 hours

hard-drive 7.6a

To reactivate bacteria from cryo stocks, first add a precise volume of the cryo stock to a shake flask. Incubate the flask to revive the dormant bacteria and allow them to multiply, thereby creating a starter culture of active cells.

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Choose between overnight and same-day reactivation of cells in cryo stocks

square-1 Overnight incubation helps activate cells and prepare starter cultures. We recommend this approach.

square-2 Same-day starter culture is also possible if a higher seeding ratio is used.

square-1 Overnight starter cultures from cryo stocks

Get starter culture provides a powerful approach to tune the seeding ratio for a High-quality culture when preparing an overnight culture. With this approach it is possible to get extremely consistent starter cultures.

square-2 Same-day starter culture from cryo stock

Usually it is possible to get a starter culture within the same day if you use a relatively high seeding ratio. The step by step guide is detailed in the subsequent pages.

Proceed to Get things ready for initializing the same-day starter culture from cryo stock protocol.

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