Get things ready

You will need these items for getting a same-day starter culture from a cryo stock.

Hardware

• BactoBox® setup including a flow cell.

• Vortex mixer.

• Incubator shaker with clamps for 250 mL shake flask

• Rack for holding 15 mL centrifuge vials.

• P20 and P200 pipette. Alternatively, P10 and P100 pipettes may also be used.

• Sterile pipette tips.

• Permanent marker.

• Sterile, serological pipettes. Preferably 10 mL version.

• Serological pipet controller.

• Sterile 5 mL centrifuge vials (or similar) for collecting and disaggregating samples.

Reagents

• 1 × cryo stock of your bacterial species.

• 1 × shake flask with growth medium. E.g. 250 mL flask with 50 mL growth medium. Preferably filtered through a 0.2 µm filter to minimize contribution of background particulates from growth medium.

• Dilution vials either ready-to-use or freshly prepared using the dilution dispenser.

• Disinfection vials.

Summary

With these things ready, proceed to Inoculate culture.