> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-cryo-stock/get-things-ready.md).

# Get things ready

You will need these items for getting a same-day starter culture from a cryo stock.

## Hardware

* [BactoBox® setup](/item-register/bactobox-r/bactobox-r.md) including a [flow cell](/item-register/consumables/flow-cell.md).
  * HW: [7.6a](/item-register/bactobox-r/bactobox-r-hardware-changelog.md)
  * SW: ≥ v2026.02a
* Vortex mixer.
* Incubator shaker with clamps for 250 mL shake flask
* Rack for holding 15 mL centrifuge vials.
* P20 and P200 pipette. Alternatively, P10 and P100 pipettes may also be used.
* Sterile pipette tips.
* Permanent marker.
* Sterile, serological pipettes. Preferably 10 mL version.
* Serological pipet controller.
* Sterile 5 mL centrifuge vials (or similar) for collecting and disaggregating samples.

## Reagents

* 1 × cryo stock of your bacterial species.
* 1 × shake flask with growth medium. E.g. 250 mL flask with 50 mL growth medium. Preferably filtered through a 0.2 µm filter to [minimize contribution of background particulates from growth medium](https://help.sbtinstruments.com/custom/advanced-sample-preparation/focus-on-target-objects).
* [Dilution vials](/item-register/vials-flasks-and-liquids/dilution-vial.md) either ready-to-use or freshly prepared using the [dilution dispenser](/item-register/accessories/dilution-dispenser.md).
* [Disinfection vials](/item-register/vials-flasks-and-liquids/disinfection-vial.md).

## Summary

With these things ready, proceed to [Inoculate culture](/mpd/workflows/get-starter-cultures/from-cryo-stock/inoculate-culture.md).


---

# Agent Instructions
This documentation is published with GitBook. GitBook is the documentation platform designed so that both humans and AI agents can read, navigate, and reason over technical content effectively. Learn more at gitbook.com.

## Querying This Documentation
If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question.

Perform an HTTP GET request on the current page URL with the `ask` query parameter, and the optional `goal` query parameter:

```
GET https://help.sbtinstruments.com/mpd/workflows/get-starter-cultures/from-cryo-stock/get-things-ready.md?ask=<question>&goal=<endgoal>
```

`ask` is the immediate question: it should be specific, self-contained, and written in natural language.
`goal` is optional and describes the broader end goal you are ultimately trying to accomplish on behalf of the user. GitBook uses it to tailor the answer towards what is most useful for that goal.

The response will contain a direct answer to the question and relevant excerpts and sources from the documentation.

Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.