# Get things ready

You will need these items for getting a same-day starter culture from a cryo stock.

## Hardware

* [BactoBox® setup](https://help.sbtinstruments.com/encyclopedia/item-register/bactobox-r#bactobox-r-setup) including a [flow cell](https://help.sbtinstruments.com/encyclopedia/item-register/consumables/flow-cell).
  * HW: [7.6a](/encyclopedia/item-register/bactobox-r/bactobox-r-hardware-changelog.md) 
  * SW: [v2026.02a](/encyclopedia/software.md)
* Vortex mixer.
* Incubator shaker with clamps for 250 mL shake flask
* Rack for holding 15 mL centrifuge vials.
* P20 and P200 pipette. Alternatively, P10 and P100 pipettes may also be used.
* Sterile pipette tips.
* Permanent marker.
* Sterile, serological pipettes. Preferably 10 mL version.
* Serological pipet controller.
* Sterile 5 mL centrifuge vials (or similar) for collecting and disaggregating samples.

## Reagents

* 1 × cryo stock of your bacterial species.
* 1 × shake flask with growth medium. E.g. 250 mL flask with 50 mL growth medium. Preferably filtered through a 0.2 µm filter to [minimize contribution of background particulates from growth medium](https://help.sbtinstruments.com/protocols/prepare-your-sample/focus-on-target-objects).
* [Dilution vials](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/dilution-vial) either ready-to-use or freshly prepared using the [dilution dispenser](https://help.sbtinstruments.com/encyclopedia/item-register/accessories/dilution-dispenser).
* [Disinfection vials](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/disinfection-vial).

## Summary

With these things ready, proceed to [Inoculate culture](/mpd/workflows/get-starter-cultures/from-cryo-stock/inoculate-culture.md).


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