# Inoculate culture

First step to get a starter culture from a cryo stock is to inoculate the growth medium.

## Step by step

{% stepper %}
{% step %}
**Thaw a cryo stock.**

Allow the cryo vial to thaw at room temperature.
{% endstep %}

{% step %}
**Inoculate**

Once thawed, add 1 mL cryo stock to 50 mL growth medium in a shake flask for a \~2% (v/v) seeding.
{% endstep %}

{% step %}
**Swirl**

Swirl the bottle for 10 seconds to disperse the cryo stock in the cultivation medium.
{% endstep %}
{% endstepper %}

## Summary

Once the growth medium is inoculated, proceed to [Determine initial concentration](/mpd/workflows/get-starter-cultures/from-cryo-stock/determine-initial-concentration.md).


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