Inoculate culture

First step to get a starter culture from a cryo stock is to inoculate the growth medium.

Step by step

1

Thaw a cryo stock.

Allow the cryo vial to thaw at room temperature.

2

Inoculate

Once thawed, add 1 mL cryo stock to 50 mL growth medium in a shake flask for a ~2% (v/v) seeding.

3

Swirl

Swirl the bottle for 10 seconds to disperse the cryo stock in the cultivation medium.

Summary

Once the growth medium is inoculated, proceed to Determine initial concentration.