> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/workflows/mpd1/get-things-ready.md).

# Get things ready

You will need these items for the protocol.

<figure><img src="/files/VhFrDE4rwBn6NmDkaC4t" alt=""><figcaption></figcaption></figure>

## Hardware

* [BactoBox® setup](https://help.sbtinstruments.com/items/bactobox-r#bactobox-r-setup) including a [flow cell](https://help.sbtinstruments.com/items/consumables/flow-cell).
  * HW: [7.6a](/item-register/bactobox-r/bactobox-r-hardware-changelog.md)
  * SW: [v2026.02a](https://help.sbtinstruments.com/software/)
* Incubator shaker
* Vortex mixer
* Rack for holding 5 and 15 mL centrifuge vials.
* Cold rack for holding 5 and 15 mL centrifuge vials, e.g. [ice rack](https://www.sigmaaldrich.com/DK/en/product/sigma/baf189050050), [chillblock](https://www.sigmaaldrich.com/DK/en/product/sigma/cls432061), or [mini cooler](https://eu.raptorsupplies.com/pd/globe-scientific/454005). You can also use a simple expanded polystyrene with a mix of water and ice (or a cooling element).
* Empty 2, 5, or 15 mL centrifuge vials for sampling.
* Serological pipette, preferably 2 mL version
* Serological pipette controller
* P200 pipette.
* Sterile pipette tips.

## Reagents

* 1 × culture in deceleration phase. Preferably use a bioreactor culture. Alternatively, you can also use a 50 mL shake flask culture in 250 mL vessel to simulate the process.
* [Dilution vials](https://help.sbtinstruments.com/items/vials-flasks-and-liquids/dilution-vial) either ready-to-use or freshly prepared using the [dilution dispenser](https://help.sbtinstruments.com/items/accessories/dilution-dispenser).
* [Disinfection vials](https://help.sbtinstruments.com/items/vials-flasks-and-liquids/disinfection-vial).

## Summary

With these things in hand, you are ready to [Create measurement group](/mpd/workflows/mpd1/create-measurement-group.md).


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