Track hourly

Step by step
Summary
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Continue hourly measurements throughout the deceleration and stationary phases until the cell concentration stabilizes. We will detail what stable means on next page.
The sample workup is identical to the step by step guide provided in Track deceleration phase.

Vortex the vial 1 minute at maximum speed to disaggregate cell clumps. See Break up clumps and chains if your bacterium needs more aggressive disaggregation than this.
Transfer the tubing kit to the 1:10 000 vial.
Press Measure in Access to start a new measurement.
Dilution: 10 000
Label: staph-epi-harvest or similar.
After completing the measurement, Access will automatically calculate the cell concentration in the culture in the field Cells/mL × Dil.
Stop the measurements when the relative change is <5% two consecutive measurements. We will detail the calculations in Conclude engineering run.
You tracked the cultures hourly throughout deceleration and stationary phase with triplicate repeats. In the next step, Conclude engineering run, you will decide when to conclude the experiment.
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