> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/mpd/workflows/track-growth-curve/get-things-ready.md).

# Get things ready

You will need these items for the growth curve experiment.

## Hardware

* [BactoBox® setup](/item-register/bactobox-r/bactobox-r.md) including a [flow cell](/item-register/consumables/flow-cell.md) with at least 50 measurements.
  * HW: [7.6a](/item-register/bactobox-r/bactobox-r-hardware-changelog.md)
  * SW: ≥ v2026.02a
* Windows 10/11 computer.
* Incubator shaker.
* Vortex mixer.
* Pipettes and sterile pipette tips: P1000, P200, P20.
* Serological pipettes. Preferably 10 and 2 mL versions.
* Serological pipet controller.
* Sterile 50 mL centrifuge vial (or similar) for disaggregating overnight culture.
* 2 mL eppendorf vials (or similar) for individual sampling points.

## Reagents

* 1 <sup><sub>x<sub></sup> [high-quality culture](/mpd/cell-growth/high-quality-culture.md).
* 1 <sup><sub>x<sub></sup> Erlenmeyer flask with cultivation medium.
* [Dilution vials](/item-register/vials-flasks-and-liquids/dilution-vial.md) either ready-to-use or freshly prepared using the [dilution dispenser](/item-register/accessories/dilution-dispenser.md).
* [Disinfection vials](/item-register/vials-flasks-and-liquids/disinfection-vial.md).

<figure><img src="/files/h9ENbjr1K6hG8bpccJfi" alt=""><figcaption></figcaption></figure>

{% hint style="success" %}

#### Use proper aseptic technique

In the example videos, we use a simple lab bench. Always use proper aseptic techniques, including sterile pipette tips, laminar air flow (LAF), and disinfection of the work environment.
{% endhint %}

Next, [create a measurement group](/mpd/workflows/track-growth-curve/create-measurement-group.md).


---

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