# Get things ready

You will need these items for the growth curve experiment.

## Hardware

* [BactoBox® setup](https://help.sbtinstruments.com/encyclopedia/item-register/bactobox-r#bactobox-r-setup) including a [flow cell](https://help.sbtinstruments.com/encyclopedia/item-register/consumables/flow-cell) with at least 50 measurements.
  * HW: [7.6a](/encyclopedia/item-register/bactobox-r/bactobox-r-hardware-changelog.md) 
  * SW: [v2026.02a](/encyclopedia/software.md)
* Windows 10/11 computer.
* Incubator shaker.
* Vortex mixer.
* Pipettes and sterile pipette tips: P1000, P200, P20.
* Serological pipettes. Preferably 10 and 2 mL versions.
* Serological pipet controller.
* Sterile 50 mL centrifuge vial (or similar) for disaggregating overnight culture.
* 2 mL eppendorf vials (or similar) for individual sampling points.

## Reagents

* 1 <sup><sub>x<sub></sup> [high-quality culture](/mpd/encyclopedia/high-quality-culture.md).
* 1 <sup><sub>x<sub></sup> Erlenmeyer flask with cultivation medium.
* [Dilution vials](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/dilution-vial) either ready-to-use or freshly prepared using the [dilution dispenser](https://help.sbtinstruments.com/encyclopedia/item-register/accessories/dilution-dispenser).
* [Disinfection vials](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/disinfection-vial).

<figure><img src="/files/h9ENbjr1K6hG8bpccJfi" alt=""><figcaption></figcaption></figure>

{% hint style="success" %}

#### Use proper aseptic technique

In the example videos, we use a simple lab bench. Always use proper aseptic techniques, including sterile pipette tips, laminar air flow (LAF), and disinfection of the work environment.
{% endhint %}

Next, [create a measurement group](/mpd/workflows/track-growth-curve/create-measurement-group.md).


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