> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/not-in-the-new-structure/measure-overnight-culture/get-things-ready.md).

# Get things ready

All the below items are required for the tutorial. Most items are supplied by SBT in a bundle.

Note that the Vitroids**™** discs must be procured from Sigma/Merck. The additional items are commonly used in microbiology laboratories.

<figure><img src="https://4216107837-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FeYTtd7c0A325muFr5lMW%2Fuploads%2FbagILsADKYz0dPASCmUd%2Fget-things-ready.JPG?alt=media&#x26;token=685eaa6e-c936-4fc6-be07-05378c07f786" alt=""><figcaption></figcaption></figure>

## Hardware

* [BactoBox® setup](/item-register/bactobox-r/bactobox-r.md) including a [flow cell](/item-register/consumables/flow-cell.md) with at least one remaining measurements.
  * HW: [7.6a](https://help.sbtinstruments.com/not-in-the-new-structure/measure-overnight-culture/spaces/AjOYV4v43T0TFlSRqgAp/pages/3JJDRTohjXVJqe8Q6JDr#v7.6a)
  * SW: ≥ v2026.02a
* Marker (not supplied)
* Vortex mixer (not supplied)
* Rack for holding 15 mL centrifuge vials (not supplied)
* Centrifuge vial, 15 mL (supplied) or 5 mL (not supplied)
* P200 pipette (not supplied)
* Sterile pipette tips (not supplied).
* Serological pipette, preferably 2 or 5 mL (not supplied).
* Serological pipette controller (not supplied).
* Shaking incubator (not supplied).

## Reagents

* Certified reference material (CRM): [*Escherichia coli* WDCM 00013 Vitroids<sup>™</sup>](https://www.sigmaaldrich.com/DK/en/product/sial/vt000137) (not supplied).
* 250 mL shake flask with 50 mL autoclaved LB medium. Preferably [baffled shake flask with vented cap](https://pipette.com/dwk-life-sciences-llc-354239.html).
* 2 × [dilution vials](/item-register/individual-items/dilution-vial.md) ready-to-use.
* 1 × [disinfection vial](/item-register/consumables/disinfection-vial.md).

## Summary

With these things in hand, you are ready to for the next step: [Inoculate LB medium](/not-in-the-new-structure/measure-overnight-culture/inoculate-lb-medium.md).


---

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