> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/not-in-the-new-structure/qualification.md).
# Qualification
The qualification procedure checks that the BactoBox® setup works as intended.
In this step by step guide we use the ready-made QC vial and Dilution vial.
## Get things ready
You need these items:
* 1 × [BactoBox®](/item-register/bactobox-r/bactobox-r.md) setup
* 1 × [Disinfection vial](/item-register/consumables/disinfection-vial.md)
* 1 × [Dilution vial](/item-register/individual-items/dilution-vial.md)
* 1 × QC vial
## Step by step
{% stepper %}
{% step %}
### Get a QC vial
Cut out only one vial at a time.
{% endstep %}
{% step %}
### Start the *QC test* program
Press and hold Measure until the *Programs* menu appears.
Press Measure again briefly to select the *QC test* program. In the QC program Measure works as the OK action from the lower bar on the BactoBox® screen.
Use the buttons to set the target concentration as shown in the video. Find the value on the center of the sticker.
{% endstep %}
{% step %}
### Clean the setup
Transfer the tubing kit to the disinfection vial.
Press OK to start the *Clean* program.
{% hint style="info" %}
If you already followed [Clean your setup](/more/how-to-guides/clean-your-setup.md), BactoBox® automatically skips this step.
{% endhint %}
{% endstep %}
{% step %}
### Measure on QC vial
Shake the vial vigorously for 10 seconds. The beads settle over time, so shaking is needed to make the suspension even.
* Transfer the tubing kit to the QC vial.
* Press Measure (OK) to start the measurement.
* Press Measure (OK) to proceed from the result screen.
{% endstep %}
{% step %}
### Clean the setup
Transfer the tubing kit to the disinfection vial and press Measure (OK) to clean. This rinses out residual silica beads.
{% endstep %}
{% step %}
### Measure on dilution vial
Use the dilution vial with nothing added to it. Transfer the tubing kit to the dilution vial and press Measure (OK) to proceed.
{% endstep %}
{% step %}
### That is it!
You get the :check-double: screen when both QC vial and Dilution vial meet the required specification. Your setup has now successfully completed the qualification and the setup is ready for your first bacterial measurements.
{% hint style="info" %}
**Discard the QC vial and the dilution vial**. They are for *single use* only.
{% endhint %}
{% endstep %}
{% endstepper %}
## When to qualify
Qualify your BactoBox® in these cases:
* **At least once a year.**
* When you relocate your BactoBox® setup.\ Example: You install your BactoBox® for the very first time, you ship it to another physical location, or you get it back from service or repair at SBT.
See [Maintenance schedule](/maintenance/maintenance-schedule.md) for more information.
{% hint style="danger" %}
### Did errors occur?
If an error occurs, or if **total/mL** is outside the limits, the program returns to the previous step to repeat the QC test with fresh reagents.
See [QC vial fails](/troubleshooting/not-in-the-new-structure/i-get-error-40xx.md) for a troubleshooting guide.
{% endhint %}
---
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