# Protocols - [Best practices](https://help.sbtinstruments.com/protocols/best-practices.md): How to take care of your BactoBox® and get the most out of it. - [Clean](https://help.sbtinstruments.com/protocols/best-practices/clean-the-setup.md) - [Deep clean](https://help.sbtinstruments.com/protocols/best-practices/deep-clean-1.md) - [Connect to Access](https://help.sbtinstruments.com/protocols/best-practices/connect-to-access.md) - [Update BactoBox® software](https://help.sbtinstruments.com/protocols/best-practices/update-bactobox-r-software.md): We continuously improve the software for your BactoBox® - [Prepare your sample](https://help.sbtinstruments.com/protocols/prepare-your-sample.md) - [Break up clumps and chains](https://help.sbtinstruments.com/protocols/prepare-your-sample/break-up-clumps-and-chains.md) - [Disaggregate mechanically](https://help.sbtinstruments.com/protocols/prepare-your-sample/break-up-clumps-and-chains/disaggregate-mechanically.md) - [Disaggregate with surfactant](https://help.sbtinstruments.com/protocols/prepare-your-sample/break-up-clumps-and-chains/disaggregate-with-surfactant.md) - [Avoid cell lysis](https://help.sbtinstruments.com/protocols/prepare-your-sample/break-up-clumps-and-chains/avoid-cell-lysis.md): Sample over time to investigate effectiveness and absence of cell lysis - [Remove large particles](https://help.sbtinstruments.com/protocols/prepare-your-sample/remove-large-particles.md) - [Hit the right concentration](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration.md) - [Dilute 1:10](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/dilute-1-10.md) - [Dilute 1:100](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/dilute-1-100.md) - [Dilute 1:1 000](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/dilute-1-1-000.md) - [Dilute 1:10 000](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/dilute-1-10-000.md) - [Dilute 1:100 000](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/dilute-1-100-000.md) - [Concentrate your sample](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/concentrate-your-sample.md): It is generally not recommended to use BactoBox® for measuring samples with very low bacterial concentrations. This page is a collection of tips and tricks for how to do so. - [Filter-centrifuge your sample](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/concentrate-your-sample/filter-centrifuge-your-sample.md) - [Filter-centrifuge your sample with Vivaspin® 20](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/concentrate-your-sample/filter-centrifuge-your-sample/filter-centrifuge-your-sample-with-vivaspin-r-20.md) - [Centrifuge your sample](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-concentration/concentrate-your-sample/centrifuge-your-sample.md) - [Hit the right conductivity](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-conductivity.md) - [Make diluent](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-conductivity/make-diluent.md): The Do-It-Yourself (DIY) guide to make diluent - [Measure conductivity with BactoBox®](https://help.sbtinstruments.com/protocols/prepare-your-sample/hit-the-right-conductivity/measure-conductivity-with-bactobox-r.md) - [Focus on target objects](https://help.sbtinstruments.com/protocols/prepare-your-sample/focus-on-target-objects.md) - [Measure particulates in growth medium](https://help.sbtinstruments.com/protocols/prepare-your-sample/focus-on-target-objects/measure-particulates-in-growth-medium.md) - [Mitigate high background](https://help.sbtinstruments.com/protocols/prepare-your-sample/focus-on-target-objects/mitigate-high-background.md) - [Make sample vial](https://help.sbtinstruments.com/protocols/prepare-your-sample/make-sample-vial.md) - [Do it yourself (DIY)](https://help.sbtinstruments.com/protocols/do-it-yourself-diy.md) - [DIY dilution vial](https://help.sbtinstruments.com/protocols/do-it-yourself-diy/assemble-dilution-dispenser.md) - [Assemble dilution dispenser](https://help.sbtinstruments.com/protocols/do-it-yourself-diy/assemble-dilution-dispenser/assemble-dilution-dispenser.md) - [Make DIY dilution vial](https://help.sbtinstruments.com/protocols/do-it-yourself-diy/assemble-dilution-dispenser/make-diy-dilution-vial.md) - [Replace syringe filter](https://help.sbtinstruments.com/protocols/do-it-yourself-diy/assemble-dilution-dispenser/replace-syringe-filter.md) - [Maintenance](https://help.sbtinstruments.com/protocols/maintenance.md): Our recommendations to take care of your BactoBox® - [Check engine light](https://help.sbtinstruments.com/protocols/maintenance/check-engine-light.md): Just like your car, BactoBox® has a check engine light - [Deep clean explained](https://help.sbtinstruments.com/protocols/maintenance/deep-clean.md) - [Shipping and storage](https://help.sbtinstruments.com/protocols/maintenance/shipping-and-storage.md) --- # Agent Instructions: Querying This Documentation If you need additional information that is not directly available in this page, you can query the documentation dynamically by asking a question. Perform an HTTP GET request on the current page URL with the `ask` query parameter: ``` GET https://help.sbtinstruments.com/protocols.md?ask= ``` The question should be specific, self-contained, and written in natural language. The response will contain a direct answer to the question and relevant excerpts and sources from the documentation. Use this mechanism when the answer is not explicitly present in the current page, you need clarification or additional context, or you want to retrieve related documentation sections.