# Deep clean

## Get things ready

You need these items:

* 1 × [BactoBox® setup](https://help.sbtinstruments.com/encyclopedia/item-register/bactobox-r#bactobox-r-setup)
* 1 × [deep clean vial](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/deep-clean-vial)

## Step by step

{% stepper %}
{% step %}

### Switch to the deep clean vial

Transfer the tubing kit to the deep clean vial.
{% endstep %}

{% step %}

### Start the *Deep clean* program

Press and hold <kbd><mark style="background-color:purple;">Measure<mark style="background-color:purple;"></kbd> for 2 seconds to enter the *Programs* menu.

Use the navigation buttons (<kbd>OK</kbd> <kbd>←</kbd> <kbd>∨</kbd> <kbd>∧</kbd>) to select the *Deep clean* program.

Press <kbd>OK</kbd> to continue.
{% endstep %}

{% step %}

### Select duration

Use the navigation buttons (<kbd>OK</kbd> <kbd>←</kbd> <kbd>∨</kbd> <kbd>∧</kbd>) to select the *Deep clean* duration.

<figure><img src="https://4216107837-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FeYTtd7c0A325muFr5lMW%2Fuploads%2Fi8DD8cQUrnpO72yIT2Bj%2Fselect-deep-clean-duration.png?alt=media&#x26;token=df243571-3dfa-49b2-9c01-c631ab491b64" alt=""><figcaption></figcaption></figure>

Press <kbd>OK</kbd> to start the *Deep clean* program.
{% endstep %}

{% step %}

### That is it!

The *Deep clean* program is done. **Discard the deep clean vial.** It is for *single use* only.

{% hint style="success" %}
Perform a [QC test](https://help.sbtinstruments.com/getting-started/qc-test) after the *Deep clean* program is done.
{% endhint %}
{% endstep %}
{% endstepper %}

## When to deep clean

[Deep clean](https://help.sbtinstruments.com/protocols/best-practices/deep-clean-1) your BactoBox® in these cases:

* **At least once a week.** Use the 15-minute option.
* To recover a flow cell that failed the QC test.\ <mark style="color:$info;">Try with the 15-minute option first. If the issue persists, try the 10-hour option.</mark>

See [maintenance](https://help.sbtinstruments.com/protocols/maintenance "mention") for more information.

## Want to know more

See [deep-clean](https://help.sbtinstruments.com/protocols/maintenance/deep-clean "mention") for additional detail.


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