# Assemble dilution dispenser

<img src="/files/etyyyj5RxAbLNZ6pnLtZ" alt="" class="gitbook-drawing">

This page shows you how to assemble a [dilution dispenser](/encyclopedia/item-register/accessories/dilution-dispenser.md).

## Quick clip :zap:

<figure><img src="/files/7EkVXsz2zsqeI7pgZXUs" alt="" width="375"><figcaption></figcaption></figure>

## Get things ready

<div align="center" data-full-width="false"><figure><img src="/files/Vjv6kQoEfjGP0q0DfTuN" alt="" width="467"><figcaption></figcaption></figure></div>

You need these items:

* 1 × [diluent](/encyclopedia/item-register/vials-flasks-and-liquids/dilution-liquid-5-l.md) (A)\
  Shipped in a separate cardboard box.
* 1 × [dilution dispenser](/encyclopedia/item-register/accessories/dilution-dispenser.md) package that includes:
  * Dispenser head (B)
  * Suction tube (C)
  * Recirculation tube (D)
  * Ejection unit (E)\
    Includes Luer lock attachment
  * 5 L adapter ring (F)\
    This is already mounted to the dispenser head (B) by SBT.
* 1 × [syringe filter](/encyclopedia/item-register/consumables/syringe-filter.md) (G)\
  Bundled in a separate box (`SF01`) that contains 40 pieces.

<figure><img src="/files/9ZzXSIXhBdjlVWC0IQe5" alt="" width="563"><figcaption><p>From left to right: <a href="/pages/Gwj4PKMJ8zzvFY1lgTy5">Dilution dispenser</a> package, <a href="/pages/sFf0TVuIzmS4MNa8cIQ3">dilution liquid</a>, and <a href="/pages/51qzHw9fIhk3wWEtbgMw">syringe filter</a> package (<code>SF01</code>).</p></figcaption></figure>

{% hint style="danger" %}

### Do not use a long, sharp knife to open the box with diluent

The knife may accidentally cut all the way through the foam padding and into the container with dilution liquid.
{% endhint %}

## Step by step

{% stepper %}
{% step %}

#### Assemble ejection unit

Insert the ejection unit (E) into the ejection valve on the dispenser head (B).

Then, turn the union nut clockwise until it is firmly in place.

<figure><img src="/files/WW3Wa7V9ocRZBR1rwvzt" alt=""><figcaption></figcaption></figure>
{% endstep %}

{% step %}

#### Assemble suction tube on dispenser valve

Slightly pull back the union nut from the suction tube (C) as shown in the image below.

Push the suction tube in until the plastic collar reaches the stop.

Turn the union nut clockwise to tighten onto the valve mantle.

<figure><img src="/files/4VilR8rLWnAwfFmR1Oiy" alt=""><figcaption></figcaption></figure>
{% endstep %}

{% step %}

#### Install recirculation tube

Insert the small recirculation tube (D) firmly into the designated hole on the dispenser head (B).

Ensure that the nozzle faces away from the suction tube (C).

![](/files/J6uMT1O2g0mSjBpuJhbr)
{% endstep %}

{% step %}

#### Install syringe filter

Screw the syringe filter (G) onto the Luer lock adapter of the ejection unit (E).

<figure><img src="/files/i376d339tk7VCPiArtDq" alt="" width="188"><figcaption></figcaption></figure>
{% endstep %}

{% step %}

#### That is it!

You are now ready to [make a DIY dilution vial](/protocols/do-it-yourself-diy/assemble-dilution-dispenser/make-diy-dilution-vial.md).
{% endstep %}
{% endstepper %}

{% hint style="info" %}
Consult the included Hirschmann manual for comprehensive details on operation, safety, and troubleshooting.
{% endhint %}


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