Dilute 1:1 000

Get two dilution vials. Discard 1 mL of diluent of the second dilution vial, so that the second dilution vial is filled to the 9 mL mark.

Dilute 1:100 into your first Dilution vial, and then Dilute 1:10 into your second dilution vial.

Vortex each dilution step thoroughly!

Always vortex thoroughly for at least 10 seconds. Ensure that a clear cyclone/vortex appears.

You must perform proper vortexing to ensure that the transferred bacteria are well-mixed in the prepared dilution. Without proper vortexing, you may get underestimation of concentration and variation between replicates.

Preparing a dilution series with 1:100, 1:1,000 and 1:10,000 dilutions

The most convenient way of preparing three samples diluted by 1:100, 1:1,000 and 1:10,000 is listed below.

Sample #1 - target dilution 1:100: Dilute 1:100 from primary sample.

Sample #2 - target dilution 1:1,000: Dilute 1:10 from sample #1. Also, see Dilute 1:1 000

Sample #3 - target dilution 1:10,000: Dilute 1:100 from sample #1. Also, see Dilute 1:10 000