# Dilute 1:10

Get a [dilution vial](https://help.sbtinstruments.com/encyclopedia/item-register/vials-flasks-and-liquids/dilution-vial). Discard 1 mL of diluent so that the dilution vial is filled to the 9 mL mark.

Add 1 mL of your sample to the dilution vial (which brings it back up to the 10 mL mark). This results in a 1:10 dilution of your sample. Once mixed together, we call it your *sample vial* from now on.

![Procedure to make 1:10 dilutions](https://4216107837-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FeYTtd7c0A325muFr5lMW%2Fuploads%2FA5jWZSaC6OmkGOxlrnW2%2F5.png?alt=media)

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## Vortex each dilution step thoroughly!

Always vortex thoroughly for at least **10 seconds**. Ensure that a clear cyclone/vortex appears.

You must perform proper vortexing to ensure that the transferred bacteria are well-mixed in the prepared dilution. Without proper vortexing, you may get underestimation of concentration and variation between replicates.
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## Your sample vial is for *single use* only

Once you run the liquid through BactoBox®, the properties change *significantly*.

**Always discard your sample vial after use!**
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## Is the final dilution factor in your sample vial 1:10?

You might run into conductivity-related errors. Avoid these with a [custom diluent](https://help.sbtinstruments.com/protocols/hit-the-right-conductivity#i-dilute-1-10-or-less).


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