Dilute 1:100 000

In the example below, the end-user had a concentrated fermentation that resulted in a concentrated end-product. This required a 1:100 000 dilution of the end product before the BactoBox® measurement.

The end-product is viscous and therefore slightly difficult to pipette reliably with a P200 pipette. To get around this, we start with a 1:10 dilution followed by two sequential 1:100 dilutions (10 × 100 × 100 = 100,000). Once mixed together, we call it your sample vial from now on.

Serial dilution for making a 1:100,000 dilution

Once you get the hang of the screening procedure, it is snappy to do the dilutions.

Viscous liquid can be difficult to pipette accurately. If this is the case, use a weight to help you. Place the 9 mL of sample on the weight and use the weight as guide while you pipette the sample into the vial.

Vortex each dilution step thoroughly!

Always vortex thoroughly for at least 10 seconds. Ensure that a clear cyclone/vortex appears.

You must perform proper vortexing to ensure that the transferred bacteria are well-mixed in the prepared dilution. Without proper vortexing, you may get underestimation of concentration and variation between replicates.

Your sample vial is for single use only

Once you run the liquid through BactoBox®, the properties change significantly.

Always discard your sample vial after use!