Experimental features may change, break, or be removed in future updates.
CIZE (Cell-Impedance-derived siZe Estimate) is the average electrical diameter of cells. It is primarily driven by cell size. Other factors may influence CIZE, such as cell morphology and electrical phenotype.
To get a CIZE value, the cells/mL concentration must be above the limit of detection.
Known uncertainties about the CIZE metric
It is early days for the CIZE metric. We know that:
For the same sample, CIZE may change from BactoBox® to BactoBox®
For the same sample, CIZE may change from flow cell to flow cell
CIZE may depend on the electrical conductivity of your sample
CIZE best practices
Stick to a single BactoBox® device and a single flow cell. Ensure that the electrical conductivity of your sample is as consistent as possible.
This ensures that you get consistent CIZE results for your sample.
Labels
Use labels to compare growth curves. Each label shows two traces: concentration (solid line) on the primary y-axis and CIZE (dashed line) on the secondary y-axis.
All measurements with an empty Label column correspond to the Unlabelled trace.
Example growth-curve measurement group. It contains two E. coli growth curves: one in CD medium and one in LB medium. Plots are grouped by label. Concentration (solid line) uses the primary y-axis (left). CIZE (dashed line) uses the secondary y-axis (right).
