> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/technical-documentation/gating/legacy-gating-settings.md).

# Legacy gating settings

{% hint style="danger" %}

### Contact SBT before you change gating

Gating is an advanced feature. Never change it without first agreeing it with SBT. The gating files on your BactoBox® have pitfalls. This is true even for a file named after the organism you work with. We only support gating as part of a [custom solution](/advanced/what-is-a-custom-solution.md). [Contact us](https://sbtinstruments.com/contact).
{% endhint %}

Over the years, we built a selection of gating settings for specific use cases. E.g., gating that targets a specific bacterial genus at a specific activity level.

Nowadays, we recommend BacTotal-10 for all use cases. We keep the other gating settings around for backward compatibility.

{% hint style="success" %}

### Use [BacTotal-10](/technical-documentation/gating/bactotal-10.md) if in doubt

BacTotal-10 fits most use cases. It is also the default gating for BactoBox®.

The other gating settings are for legacy purposes.
{% endhint %}

## List of legacy gating settings

<table data-full-width="false"><thead><tr><th width="233">Gating</th><th>Works on my BactoBox®?</th><th>Notes</th></tr></thead><tbody><tr><td>BacTotal_v2024-02</td><td><strong>Unknown.</strong> Risk of misinterpretation. Reach out to SBT before use.</td><td>Earlier version of <a href="/technical-documentation/gating/bactotal-10.md">BacTotal-10</a> for legacy use cases.<br>The default for the legacy BB05 (Original) <a href="/technical-documentation/hardware/bactobox-r-configuration.md">configuration</a>.</td></tr><tr><td>low_conductivity</td><td><strong>Unknown.</strong> Risk of misinterpretation. Reach out to SBT before use.</td><td>May underestimate small cells compared to BacTotal-10.</td></tr><tr><td>Salmonella</td><td><strong>Unknown.</strong> Risk of misinterpretation. Reach out to SBT before use.</td><td></td></tr><tr><td>Lactobacillus_rhamnosus</td><td><strong>Unknown.</strong> Risk of misinterpretation. Reach out to SBT before use.</td><td></td></tr><tr><td>Pseudomonas</td><td><strong>Unknown.</strong> Risk of misinterpretation. Reach out to SBT before use.</td><td></td></tr><tr><td>Mycoplasma</td><td><strong>Unknown.</strong> Risk of misinterpretation. Reach out to SBT before use.</td><td></td></tr><tr><td>Bacillus_endospores</td><td><strong>Unknown.</strong> Risk of misinterpretation. Reach out to SBT before use.</td><td></td></tr></tbody></table>

If SBT agrees that you should use one of these, see [Set a default gating](/advanced/gating-in-access/set-a-default-gating.md).


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