Error codes

Look up the code on your BactoBox® screen and follow the steps.

Find your code in the table. Use the column for where the error appeared, during a sample or during a QC test. Follow the steps in order, and stop as soon as the error is gone. If the last step does not fix it, contact support.

Two steps come up often.

• Clean and try again. Run Clean with a fresh SBT disinfection vial, even if your current one has uses left. Then measure a new sample vial, or run the QC test again with a fresh SBT QC vial and a fresh SBT ready-made dilution vial.

• Restart. Switch BactoBox® off and on with the rocker switch. Wait for the Home screen, then try again.

When a step says Run a QC test

Run it with SBT consumables only, so the result tells you about your BactoBox®. The result decides what comes next. If the QC test passes, your BactoBox® works and the cause is in the sample. See Sample gives an error. If it fails, follow the QC test column for the code it shows.

Use liquids at room temperature

Warm or cold liquid changes the conductivity. Make sure your dilution liquid, QC vial and dilution vial are at room temperature before you try again.

Codes

Code	Message	During a sample	During a QC test
1000	Electrical signal is too low	Clean and try again → Run a QC test	Remove and reinsert the flow cell, then clean and try again → Check the flow cell
1001	Electrical signal is too high	Clean and try again → Run a QC test	Clean and try again → Check the flow cell
1101	Electrical noise is too high	Clean and try again → Run a QC test	Clean and try again → Check the flow cell
1111	Electrical noise is too high	Clean and try again → Run a QC test	Clean and try again → Check the flow cell
1121	Electrical noise is too high	Clean and try again → Run a QC test	Clean and try again → Check the flow cell
1131	Electrical noise is too high	Clean and try again → Run a QC test	Clean and try again → Check the flow cell
1200	Conductivity is too low…	Clean and try again → Check that you use SBT dilution liquid at room temperature → Run a QC test	Check that the QC vial and dilution vial are at room temperature → Clean and try again → Check liquid uptake
1201	Conductivity is too high…	Check that your dilution liquid is at room temperature, not warm → Dilute more, for example 1:1 000, to see if the growth medium raises the conductivity → Run a QC test	Check that the QC vial and dilution vial are at room temperature → Restart
1202	Conductivity was not stable…	Clean and try again → Run a QC test	Check that the QC vial and dilution vial are at room temperature → Clean and try again → Restart
1203	Conductivity sensor is malfunctioning	Clean and try again → Restart	Clean and try again → Restart
1204	Could not read from conductivity sensor	Clean and try again → Restart	Clean and try again → Restart
1205	Conductivity is way too high…	Check that your dilution liquid is at room temperature, not warm → Dilute more, for example 1:1 000, to see if the growth medium raises the conductivity → Run a QC test	Check that the QC vial and dilution vial are at room temperature → Restart
1206	Conductivity is way too low…	Clean and try again → Check that you use SBT dilution liquid at room temperature → Run a QC test	Check that the QC vial and dilution vial are at room temperature → Clean and try again → Check liquid uptake
1207	Cleaning liquid conductivity is too high…	Appears during Clean. Clean again with a fresh SBT disinfection vial at room temperature.	Appears during Clean. Clean again with a fresh SBT disinfection vial at room temperature.
2000	Detected events are too fast	If your sample holds many particles, dilute it more. Clean and try again → Run a QC test	Clean and try again → Check the flow cell
2001	Detected events are too slow	If your sample holds many particles, dilute it more. Clean and try again → Run a QC test	Clean and try again → Check the flow cell
2004	Liquid moves slower than expected	If your sample holds many particles, dilute it more. Clean and try again → Run a QC test	Clean and try again → Check the flow cell
2006	Flow did not stabilize in time	If your sample holds many particles, dilute it more. Clean and try again → Run a QC test	Clean and try again → Check the flow cell
2010	Velocity differs between detection sites	If your sample holds many particles, dilute it more. Clean and try again → Run a QC test	Clean and try again → Check the flow cell
2020	Concentration differs between detection sites	If your sample holds many particles, dilute it more. Clean and try again → Run a QC test	Clean and try again → Check the flow cell
2030	Flow cell may be clogged	If your sample holds many particles, dilute it more. Clean and try again → Run a QC test	Clean and try again → Check the flow cell
2103	QC test required	Appears when you press Measure on a new flow cell. Run a QC test.	Does not occur
3000	Particle concentration is too high…	Dilute more. Clean and try again. See Hit the right concentration.	Does not occur
4001	No objects detected	Does not occur	Restart → Check the flow cell
4010	…total cells/mL is outside the allowed range…	Does not occur	Clean and try again → Check your consumables
4011	…particles/mL is outside the allowed range…	Does not occur	Clean and try again → Check your consumables
4012	Detected object velocities are outside normal limits	Does not occur	Clean and try again → Check the flow cell
4013	HF phase is not within…	Does not occur	Check the flow cell
4014	HF amplitude is not within…	Does not occur	Check the flow cell
4020	QC test failed…	Does not occur	Clean and try again → Check the flow cell
5010	…total cells/mL is outside the allowed range…	Does not occur	Clean and try again → Check your consumables
5011	…particles/mL is outside the allowed range…	Does not occur	Clean and try again → Check your consumables
8100	Measurement did not compute final data in time	Restart → Update BactoBox® software	Restart → Update BactoBox® software
9969	The electronics may be damaged	Restart → Update BactoBox® software	Restart → Update BactoBox® software
9970	Could not read reference file	Restart → Update BactoBox® software	Restart → Update BactoBox® software

In a QC test, codes 4001 to 4020 come from the QC vial. Codes 5010 and 5011 come from the blank, the plain dilution vial measured at the end.

The wording of a message can differ between software versions. The code stays the same. Code not listed? Contact support.

Messages without a code

Message	Go to
Program could not run to completion	Program could not run to completion
Flow cell invalid, flow cell obsolete, flow cell depleted, flow cell removed	Flow cell does not work
Update failed	Software update failed
This site can't be reached	Cannot connect to Access
Initializing, Stopping	Stuck at spinner

For other problems without a message, see Other problems.