> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/troubleshooting/error-codes.md). # Error codes Look up the code on your BactoBox® screen and follow the steps. Find your code in the table. Use the column for where the error appeared, during a sample or during a QC test. Follow the steps in order, and stop as soon as the error is gone. If the last step does not fix it, [contact support](/troubleshooting/contact-support.md). Two steps come up often. * **Clean and try again.** Run [Clean](/more/how-to-guides/clean-your-setup.md) with a fresh SBT [disinfection vial](/item-register/consumables/disinfection-vial.md), even if your current one has uses left. Then measure a new sample vial, or run the QC test again with a fresh SBT QC vial and a fresh SBT ready-made dilution vial. * **Restart.** Switch BactoBox® off and on with the rocker switch. Wait for the *Home* screen, then try again with fresh vials. {% hint style="info" %} ### When a step says *Run a QC test* Run it with SBT consumables only, so the result tells you about your BactoBox®. The result decides what comes next. If the QC test passes, your BactoBox® works and the cause is in the sample. See [Sample gives an error](/troubleshooting/sample-gives-an-error.md). If it fails, follow the QC test column for the code it shows. {% endhint %} {% hint style="success" %} ### Use fresh vials at room temperature Alcohol left inside BactoBox® after Clean can lower the conductivity, so a vial you have already measured may read too low. Warm or cold liquid also changes the conductivity. Try again with a fresh vial, and make sure your dilution liquid, QC vial and dilution vial are at room temperature. {% endhint %} ## Codes | Code | Message | During a sample | During a QC test | | ---- | ---------------------------------------------------- | --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- | ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ | | 1000 | Electrical signal is too low | Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | [Remove and reinsert the flow cell](/getting-started/installation/install-flow-cell.md), then clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 1001 | Electrical signal is too high | Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 1101 | Electrical noise is too high | Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 1111 | Electrical noise is too high | Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 1121 | Electrical noise is too high | Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 1131 | Electrical noise is too high | Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 1200 | Conductivity is too low… | Clean and try again → Check that you use SBT dilution liquid at room temperature → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Check that the QC vial and dilution vial are at room temperature → Clean and try again → [Check liquid uptake](/troubleshooting/qc-test-fails/check-liquid-uptake.md) | | 1201 | Conductivity is too high… | Check that your dilution liquid is at room temperature, not warm → Dilute more, for example 1:1 000, to see if the growth medium raises the conductivity → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Check that the QC vial and dilution vial are at room temperature → Restart | | 1202 | Conductivity was not stable… | Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Check that the QC vial and dilution vial are at room temperature → Clean and try again → Restart | | 1203 | Conductivity sensor is malfunctioning | Clean and try again → Restart | Clean and try again → Restart | | 1204 | Could not read from conductivity sensor | Clean and try again → Restart | Clean and try again → Restart | | 1205 | Conductivity is way too high… | Check that your dilution liquid is at room temperature, not warm → Dilute more, for example 1:1 000, to see if the growth medium raises the conductivity → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Check that the QC vial and dilution vial are at room temperature → Restart | | 1206 | Conductivity is way too low… | Clean and try again → Check that you use SBT dilution liquid at room temperature → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Check that the QC vial and dilution vial are at room temperature → Clean and try again → [Check liquid uptake](/troubleshooting/qc-test-fails/check-liquid-uptake.md) | | 1207 | Cleaning liquid conductivity is too high… | Appears during *Clean*. Clean again with a fresh SBT [disinfection vial](/item-register/consumables/disinfection-vial.md) at room temperature. | Appears during *Clean*. Clean again with a fresh SBT [disinfection vial](/item-register/consumables/disinfection-vial.md) at room temperature. | | 2000 | Detected events are too fast | If your sample holds many particles, dilute it more. Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 2001 | Detected events are too slow | If your sample holds many particles, dilute it more. Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 2004 | Liquid moves slower than expected | If your sample holds many particles, dilute it more. Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 2006 | Flow did not stabilize in time | If your sample holds many particles, dilute it more. Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 2010 | Velocity differs between detection sites | If your sample holds many particles, dilute it more. Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 2020 | Concentration differs between detection sites | If your sample holds many particles, dilute it more. Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 2030 | Flow cell may be clogged | If your sample holds many particles, dilute it more. Clean and try again → [Run a QC test](/maintenance/qc-test/run-qc-test.md) | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 2103 | QC test required | Appears when you press *Measure* on a new flow cell. [Run a QC test](/maintenance/qc-test/run-qc-test.md). | Does not occur | | 3000 | Particle concentration is too high… | Dilute more. Clean and try again. See [Hit the right concentration](/more/how-to-guides/hit-the-right-concentration.md). | Does not occur | | 4001 | No objects detected | Does not occur | Restart → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 4010 | …total cells/mL is outside the allowed range… | Does not occur | Clean and try again → [Check your consumables](/troubleshooting/qc-test-fails/check-your-consumables.md) | | 4011 | …particles/mL is outside the allowed range… | Does not occur | Clean and try again → [Check your consumables](/troubleshooting/qc-test-fails/check-your-consumables.md) | | 4012 | Detected object velocities are outside normal limits | Does not occur | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 4013 | HF phase is not within… | Does not occur | [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 4014 | HF amplitude is not within… | Does not occur | [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 4020 | QC test failed… | Does not occur | Clean and try again → [Check the flow cell](/troubleshooting/qc-test-fails/check-the-flow-cell.md) | | 5010 | …total cells/mL is outside the allowed range… | Does not occur | Clean and try again → [Check your consumables](/troubleshooting/qc-test-fails/check-your-consumables.md) | | 5011 | …particles/mL is outside the allowed range… | Does not occur | Clean and try again → [Check your consumables](/troubleshooting/qc-test-fails/check-your-consumables.md) | | 8100 | Measurement did not compute final data in time | Restart → [Update BactoBox® software](/more/software/update-bactobox-r-software.md) | Restart → [Update BactoBox® software](/more/software/update-bactobox-r-software.md) | | 9969 | The electronics may be damaged | Restart → [Update BactoBox® software](/more/software/update-bactobox-r-software.md) | Restart → [Update BactoBox® software](/more/software/update-bactobox-r-software.md) | | 9970 | Could not read reference file | Restart → [Update BactoBox® software](/more/software/update-bactobox-r-software.md) | Restart → [Update BactoBox® software](/more/software/update-bactobox-r-software.md) | In a QC test, codes 4001 to 4020 come from the QC vial. Codes 5010 and 5011 come from the blank, the plain dilution vial measured at the end. The wording of a message can differ between software versions. The code stays the same. Code not listed? [Contact support](/troubleshooting/contact-support.md). ## Messages without a code | Message | Go to | | ---------------------------------------------------------------------------- | ------------------------------------------------------------------------------------------------------------- | | Program could not run to completion | [Program could not run to completion](/troubleshooting/other-problems/program-could-not-run-to-completion.md) | | Flow cell invalid, flow cell obsolete, flow cell depleted, flow cell removed | [Flow cell does not work](/troubleshooting/other-problems/flow-cell-does-not-work.md) | | Update failed | [Software update failed](/troubleshooting/other-problems/software-update-failed.md) | | This site can't be reached | [Cannot connect to Access](/troubleshooting/other-problems/cannot-connect-to-access.md) | | Initializing, Stopping | [Stuck at spinner](/troubleshooting/other-problems/stuck-at-spinner.md) | For other problems without a message, see [Other problems](/troubleshooting/other-problems.md). --- # Agent Instructions This documentation is published with GitBook. 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