Dilution vial fails

Before you move on

You are here because the QC test failed. E.g., the measurement gave an error, or the concentration was outside the allowed range.

This assumes that Measure on QC vial passed!

Is this not the case? Then let's take a step back. Is it the measurement on the QC vial that failed?

See also BactoBox® acting up?.

Step by step

1

Replace the syringe filter on your dilution dispenser

Skip this step if you use the SBT dilution vial (bundled as DV01)

First of all, make sure that your dilution dispenser has a syringe filter on the outlet as shown here:

The syringe filter clogs over time and gets contaminated. In this case, replace the syringe filter as shown here:

Perform a QC test again with a new dilution vial.

2

Try with another brand of empty vial

Skip this step if you already use the SBT dilution vial (bundled as DV01)

Third-party empty vials may contain a surprising number of loose particles. Even empty vials from name brands.

When you make your dilution vial (e.g., with the dilution dispenser), use the SBT empty vial (bundled as VI06) instead.

Perform a QC test again with a new dilution vial.

3

Use an SBT dilution vial

Skip this step if you already use the SBT dilution vial (bundled as DV01)

It is tricky to make a proper dilution vial yourself. You may get errors even with a syringe filter in place and an SBT empty vial (bundled as VI06). Therefore, we offer the option to buy ready-to-use dilution vials (bundled as DV01).

Perform a QC test again with an SBT dilution vial (bundled as DV01).

SBT dilution vial

Use the SBT dilution vial (bundled as DV01)

The SBT dilution vial pairs perfectly with your BactoBox®. It avoids all the pitfalls of DIY dilution vials.

The SBT dilution vial is filtered, conductivity-adjusted, and long-term stable thanks to gamma radiation sterilization.

The dilution vial is for single use only

Discard the dilution vial after use. Once it's been through BactoBox®, we can no longer guarantee the sterility and purity.

Tried all of the above

Did you try all of the above and nothing helped?​

This is a hardware issue with your BactoBox® setup. Contact our support team to discuss the matter further. Make sure to mention it if you found:

• A leak inside the flow cell compartment

• Sign of corrosion or deterioration inside the flow cell department

• Broken or loose inlet/outlet (where you connect the tubing kit to your BactoBox®)

• Rattling or loose components

• In general, hardware damage of any kind

If possible, include pictures of the problem. This helps us help you faster!