Error about slow/fast flow

Before you move on

You went through Run QC test and your BactoBox® setup passed.

Is this not the case? Then let's take a step back. Did the QC test fail? Does your BactoBox® not even power on?

See also BactoBox® acting up?.

The measurement triggers an error about too slow/fast flow

You run the Measure program but it gives an error such as:

• detected events are too slow/fast

• liquid moves slower than expected

• concentration/velocity differs between sites

or something of that nature.

Error codes 2000–2999 (both inclusive) relate to the flow paths in your BactoBox® setup.

Step by step

1

Check for leaks

Look for droplets or moisture especially near:

• The inlet and outlet of your BactoBox® (where you connect the tubing kit).

• Inside the flow cell compartment (under the flow cell cover). Remove the flow cell to see if there are droplets or moisture underneath.

See Installation for details.

Power off your BactoBox® if you find a leak inside the flow cell compartment. Wipe off the liquid with a dry cloth. Wait for it to dry thoroughly. Perform a QC test to ensure that your BactoBox® didn't take permanent damage.

2

Check that everything is correctly in place

Ensure that the tubing kit and external filter is tightly connected to your BactoBox®.

Press down on the flow cell to ensure that is properly in place. Press down hard to be sure.

You hear two clicks during flow cell installation. Afterwards, it should just feel slightly springy when you press the center part of the flow cell (no more click sounds).

3

Replace the external filter

Replace the external filter. See Install tubing kit for details.

Perform a QC test again with your new external filter in place and a new QC vial.

4

Replace the flow cell

Replace the flow cell. See Install flow cell for details.

Perform a QC test again with your new flow cell in place and a new QC vial.

Clean the flow cell before you remove it.

What about my other flow cell that didn't work?

First try the 15-min Deep clean program on your flow cell. If the issue persists, try the 10-hour Deep clean program.

5

Check your sample

Before you measure on your own sample again, make sure that you both break up clumps and chains and remove large particles. In general, it is important to prepare your sample for use with BactoBox®.

Tried all of the above

Did you try all of the above and nothing helped?

This is a hardware issue with your BactoBox® setup. Contact our support team to discuss the matter further. Make sure to mention it if you found:

• A leak inside the flow cell compartment

• Sign of corrosion or deterioration inside the flow cell department

• Broken or loose inlet/outlet (where you connect the tubing kit to your BactoBox®)

• Rattling or loose components

• In general, hardware damage of any kind

If possible, include pictures of the problem. This helps us help you faster!