> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/tutorials/measure-vitroid-tm-disc/disaggregate-bacteria.md).

# Prepare sample

The Vitroids**™** disc is now dissolving in the diluent. You will speed up the dissolution process by vortexing the disc for one minute at maximum speed. The below video demonstrates the procedure and the details are provided in the [#step-by-step](#step-by-step "mention") instructions.

{% hint style="success" icon="bacteria" %}

## Vortex thoroughly and vigorously

BactoBox is a flow cytometer and works best when cells are present in a single-cell suspension.

Cells stick together and you need thorough disaggregation to prepare a single-cell suspension.

One minute of vortexing may sound excessive, but trust us. You need it!
{% endhint %}

## Step by step

{% stepper %}
{% step %}

### Vortex vial

Transfer the small vial to a vortex mixer and vortex at maximum speed for 1 min.\
Ensure you see a proper vortex forming in the tube.

<figure><img src="/files/atWCCqLwixhvTrDmBQ48" alt=""><figcaption><p>Use a vortex mixer to solubilize Vitroids™ disc and disaggregate bacterial clumps.</p></figcaption></figure>
{% endstep %}

{% step %}

### Inspect the vial for complete solubilization

Visually inspect the vial for residual clumps of the Vitroid&#x73;**™** disc.\
Increase vortexing time if you see clumps.\
\
The disc must be completely solubilized before proceeding to next page.
{% endstep %}
{% endstepper %}

## Summary

You have now dissolved the disc and disaggregated bacterial clumps. Proceed to [Dilute and measure](/tutorials/measure-vitroid-tm-disc/dilute-and-measure.md) for your first BactoBox® measurement on cells.


---

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