> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/tutorials/measure-vitroid-tm-disc/transfer-vitroid-tm-disc.md).
# Transfer disc
BactoBox® measures bacteria in liquid suspension. A Vitroids**™** disc is solid. Therefore you must first solubilize the disc. The below video demonstrates the procedure and all the details are provided in the [#step-by-step](#step-by-step "mention") instructions.
{% hint style="success" %}
## Bacteria can only be in BactoBox® diluent for short durations.
In this tutorial we use diluent from a [Dilution vial](/item-register/vials-flasks-and-liquids/dilution-vial.md) to solubilize the bacteria in the disc. The diluent provides excellent differentiation of cells from other objects. On the flip side it is [hypotonic ](#user-content-fn-1)[^1]and cells may lyse if stored in the diluent. Always complete measurements within 15 minutes after dilution in BactoBox® diluent.
{% endhint %}
Transfer of Vitroid™ disc to diluent for initial solubilization.
## Step by step
{% stepper %}
{% step %}
### Label a vial
Find a small vial, for example a 1.5 mL Eppendorf vial.
Use a marker to label the vial *E. coli*.
{% endstep %}
{% step %}
### Prepare a solubilization vial
Use a P200 pipette to transfer 110 µL of [Dilution vial](/item-register/vials-flasks-and-liquids/dilution-vial.md) to the small vial.
{% hint style="success" %}
Preferably use the gamma-sterilized ready-to-use [Dilution vial](/item-register/vials-flasks-and-liquids/dilution-vial.md) instead of DIY versions of the BactoBox® diluent.
{% endhint %}
{% endstep %}
{% step %}
### Transfer Vitroids**™** disc to vial
Uncap the Vitroids**™** vial and hold it over the mound of the small vial.\
Tap the Vitroids**™** vial until the disc is transferred to the small vial.\
Cap the small vial tightly.
{% endstep %}
{% step %}
### Discard the opened dilution vial
Discard the dilution vial that you used to retrieve 110 µL BactoBox® diluent.
The [Dilution vial](/item-register/vials-flasks-and-liquids/dilution-vial.md) is no longer sterile and does not contain the required 10 mL volume.
{% endstep %}
{% endstepper %}
## Summary
You have now prepared for the solubilization of the Vitroid**™** disc. The disc would dissolve by itself within 5 minutes, but in the next page we will speed up this process by vortexing. This will also disaggregate clumps of bacteria. Proceed to [Prepare sample](/tutorials/measure-vitroid-tm-disc/disaggregate-bacteria.md) for further instructions.
[^1]: A solution with a lower concentration of solutes compared to another solution (like the fluid inside a cell)
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