The below items are required for the tutorial. Most items are supplied by a SBT in a standard BactoBox® bundle.
Note that the Vitroids™ discs must be procured from Sigma/Merck. The additional items are commonly used in microbiology laboratories.
BactoBox® setup including a flow cell with at least one remaining measurements.
HW: 7.6a
SW: ≥ v2026.02a
Marker (not supplied)
Vortex mixer (not supplied)
Rack for holding 15 mL centrifuge vials (not supplied)
Centrifuge vial, 15 mL (supplied) or 5 mL (not supplied)
P200 pipette (not supplied)
Sterile pipette tips (not supplied).
Serological pipette, preferably 2 or 5 mL (not supplied).
Serological pipette controller (not supplied).
Shaking incubator (not supplied).
Countdown timer (not supplied).
Certified reference material (CRM): Escherichia coli WDCM 00013 Vitroids™ (not supplied).
250 mL shake flask with 50 mL autoclaved LB medium. Preferably baffled shake flask with vented cap.
Dilution vials ready-to-use.
Disinfection vial.
With these things in hand, you are ready to for the next step: Inoculate LB medium.
Last updated 22 days ago
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