> For the complete documentation index, see [llms.txt](https://help.sbtinstruments.com/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://help.sbtinstruments.com/item-register/what-every-sample-uses.md).

# What every sample uses

What one sample consumes, and how far each pack goes

Every sample uses **one flow cell measurement**, and most often **two dilution vials**. The two vials give two serial 1:100 steps, reaching 1:10,000.

How far you dilute depends on how many cells your sample holds, so the number of vials can vary. Two steps to 1:10,000 is the usual choice for a bacterial cultivation nearing the end of growth.

Cleaning follows your working day. Deep cleaning and the QC test follow the week.

## Per measurement

<table><thead><tr><th width="230">Item</th><th>How much</th><th width="150">Order as</th></tr></thead><tbody><tr><td><a href="/item-register/consumables/flow-cell.md">Flow cell</a></td><td>One of its 250 measurements</td><td><code>FC03</code></td></tr><tr><td><a href="/item-register/individual-items/dilution-vial.md">Dilution vial</a></td><td>Most often two, for two 1:100 steps</td><td><code>DR01</code> or <code>DB01</code></td></tr></tbody></table>

{% hint style="info" %}
Only a completed measurement counts against the flow cell. Cleaning does not, and neither does a QC test.
{% endhint %}

## Per operating day

<table><thead><tr><th width="230">Item</th><th>How much</th><th width="150">Order as</th></tr></thead><tbody><tr><td><a href="/item-register/consumables/disinfection-vial.md">Disinfection vial</a></td><td>One vial, or 10 cleans, whichever comes first</td><td><code>VI07</code></td></tr><tr><td><a href="/item-register/consumables/syringe-filter.md">Syringe filter</a></td><td>One, on the bulk dilution route only</td><td><code>SF01</code></td></tr></tbody></table>

{% hint style="info" %}
**A disinfection vial is a day, not a sample count**

Discard it at the end of the day even if you cleaned only once. You clean after every measurement, after a deep clean, and after 30 minutes of inactivity, so ten cleans can also run out before the day does.
{% endhint %}

## Per week

<table><thead><tr><th width="230">Item</th><th>How much</th><th width="150">Order as</th></tr></thead><tbody><tr><td><a href="/item-register/consumables/deep-clean-vial.md">Deep clean vial</a></td><td>One deep clean, at least once a week</td><td><code>DC02</code></td></tr><tr><td><a href="/item-register/individual-items/qc-stock.md">QC stock</a></td><td>One QC test, and one more after every deep clean</td><td><code>QC06</code></td></tr></tbody></table>

{% hint style="info" %}
**What a QC test consumes**

One dilution vial from the [QC bundle](/item-register/consumables/qc-bundle.md) to prepare the QC sample, which is a single 1:100 dilution. Then one of your day-to-day dilution vials for the blank, which you buy separately as `DR01` or `DB01`.
{% endhint %}

## How far a pack goes

Dilution coverage below assumes two vials per sample.

<table><thead><tr><th width="110">Item no.</th><th width="300">Item</th><th>Enough for</th></tr></thead><tbody><tr><td><code>FC03</code></td><td><a href="/item-register/consumables/flow-cell.md">Flow cell incl. two external filters</a></td><td>250 samples</td></tr><tr><td><code>GS01</code></td><td><a href="/item-register/consumables/getting-started-bundle.md">Getting started bundle (100× samples)</a></td><td>100 samples of dilution, 40 operating days of cleaning, 10 deep cleans and 18 QC tests. No flow cell.</td></tr><tr><td><code>DR01</code></td><td><a href="/item-register/consumables/dilution-liquid-ready-made-vials.md">Dilution liquid: ready-made vials (100× dilutions)</a></td><td>50 samples</td></tr><tr><td><code>DB01</code></td><td><a href="/item-register/consumables/dilution-liquid-bulk.md">Dilution liquid: bulk (500× dilutions)</a></td><td>250 samples</td></tr><tr><td><code>VI07</code></td><td><a href="/item-register/consumables/disinfection-vial.md">Disinfection vial (20×)</a></td><td>20 operating days</td></tr><tr><td><code>DC02</code></td><td><a href="/item-register/consumables/deep-clean-vial.md">Deep clean vial (5×)</a></td><td>5 deep cleans</td></tr><tr><td><code>QC06</code></td><td><a href="/item-register/consumables/qc-bundle.md">QC bundle (18× QC tests)</a></td><td>18 QC tests, blanks not included</td></tr><tr><td><code>SF01</code></td><td><a href="/item-register/consumables/syringe-filter.md">Syringe filter for dilution dispenser (40×)</a></td><td>40 operating days on the bulk route</td></tr></tbody></table>

{% hint style="info" %}
**Two ways to get the dilution vials**

Ready-made, or filled by you from bulk liquid.
{% endhint %}

{% content-ref url="/pages/fdKzv8gMpZm7CJHzcCfD" %}
[Dilution routes](/item-register/dilution-routes.md)
{% endcontent-ref %}


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