Prepare your sample

BactoBox® imposes some requirements for your samples. Sample preparation ensures that your sample meets these requirements.

For this purpose, we encourage you to make a sample preparation protocol. That is, a step-by-step method that makes your sample ready for BactoBox®.

Questions about your sample

If you answer yes to any of these questions, you need to address it in your sample preparation protocol! Visit the corresponding links for more information.

Does your bacteria form clumps or chains?

Streptococci, Bacillus species, and lactic acid bacteria are notorious examples.

Break up clumps and chains

Does your sample contain particulate matter?

Anything above 10 µm is large. This is usually the case for coarse samples.

Remove large particles

Does your sample have high bacterial concentration?

Anything above 5 million cells/mL is high. Usually when you want to produce bacteria.

Hit the right concentration

Does your sample have low bacterial concentration?

Anything below 0.5 million cells/mL is low. May be the case if your bacteria are still in the lag phase.

Concentrate your sample

Want to verify the absence of bacteria?

This is not the ideal use case for BactoBox®. The lower limit of detection is 30 000 cells/mL.

Application-specific protocols

To get you started, we have a selection of application-specific protocols below. Use these protocols as a starting point and tailor them to your specific application.