Dilute 1:10

Get a dilution vial. Discard 1 mL of diluent so that the dilution vial is filled to the 9 mL mark.

Add 1 mL of your sample to the dilution vial (which brings it back up to the 10 mL mark). This results in a 1:10 dilution of your sample. Once mixed together, we call it your sample vial from now on.

Procedure to make 1:10 dilutions

Vortex your sample vial

Always vortex thoroughly for at least 10 seconds. Ensure that a clear cyclone/vortex appears.

Bacteria are surprisingly good at sticking together. Without proper vortexing, you may get underestimation and variation between replicates.

Your sample vial is for single use only

Once you run the liquid through BactoBox®, the properties change significantly.

Always discard your sample vial after use!

Is the final dilution factor in your sample vial 1:10?

You might run into conductivity-related errors. Avoid these with a custom diluent.